論文 - 詳細
| RRC ID | 52336 |
|---|---|
| 著者 | Ogawa M, Kitano T, Kawata N, Sugihira T, Kitakaze T, Harada N, Yamaji R. |
| タイトル | Daidzein down-regulates ubiquitin-specific protease 19 expression through estrogen receptor β and increases skeletal muscle mass in young female mice. |
| ジャーナル | J Nutr Biochem |
| Abstract |
Ubiquitin-specific protease 19 (USP19) is a key player in the negative regulation of muscle mass during muscle atrophy. Loss-of-function approaches demonstrate that 17β-estradiol (E2) increases USP19 expression through estrogen receptor (ER) α and consequently decreases soleus muscle mass in young female mice under physiological conditions. Daidzein is one of the main isoflavones in soy, and activates ERβ-dependent transcription. Here, we investigated the effects of daidzein on E2-increased USP19 expression and E2-decreased soleus muscle mass in young female mice. Daidzein stimulated the transcriptional activity of ERβ in murine C2C12 cells and down-regulated USP19 expression. Consistently, daidzein inhibited E2-induced USP19 expression in a reporter activity using a functional half-estrogen response element (hERE) from Usp19. Daidzein inhibited E2-induced recruitment of ERα and promoted recruitment of ERβ to the Usp19 hERE. Dietary daidzein down-regulated the expression of USP19 at the mRNA and protein levels and increased soleus muscle mass in female mice, but not in males. In soleus muscle from ovariectomized (OVX) female mice, dietary daidzein inhibited E2-increased USP19 mRNA expression and E2-decreased muscle mass. Furthermore, E2 induced the recruitment of ERα and ERβ to the hERE, whereas daidzein inhibited E2-induced recruitment of ERα, and enhanced E2-increased recruitment of ERβ, to the Usp19 hERE. These results demonstrate that dietary daidzein decreases USP19 mRNA expression through ERβ and increases soleus muscle mass in young female mice, but not in male mice, under physiological conditions. |
| 巻・号 | 49 |
| ページ | 63-70 |
| 公開日 | 2017-11-1 |
| DOI | 10.1016/j.jnutbio.2017.07.017 |
| PII | S0955-2863(17)30284-X |
| PMID | 28886438 |
| MeSH | Active Transport, Cell Nucleus Animals Animals, Outbred Strains Cell Line Dietary Supplements* Endopeptidases Enzyme Repression Estrogen Receptor beta / agonists* Estrogen Receptor beta / genetics Estrogen Receptor beta / metabolism Female Genes, Reporter Isoflavones / therapeutic use* Male Mice Muscle, Skeletal / enzymology Muscle, Skeletal / metabolism* Muscle, Skeletal / pathology Myoblasts, Skeletal / cytology Myoblasts, Skeletal / enzymology Myoblasts, Skeletal / metabolism Myoblasts, Skeletal / pathology Ovariectomy / adverse effects Phytoestrogens / therapeutic use* Random Allocation Response Elements Sarcopenia / etiology Sarcopenia / metabolism Sarcopenia / pathology Sarcopenia / prevention & control* Sex Characteristics Signal Transduction Ubiquitin-Specific Proteases / antagonists & inhibitors* Ubiquitin-Specific Proteases / genetics Ubiquitin-Specific Proteases / metabolism |
| IF | 4.414 |
| 引用数 | 5 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | C2C12(RCB0987) |