論文 - 詳細
| RRC ID | 52349 |
|---|---|
| 著者 | Fukushima T, Hara-Yamamura H, Nakashima K, Tan LC, Okabe S. |
| タイトル | Multiple-endpoints gene alteration-based (MEGA) assay: A toxicogenomics approach for water quality assessment of wastewater effluents. |
| ジャーナル | Chemosphere |
| Abstract |
Wastewater effluents contain a significant number of toxic contaminants, which, even at low concentrations, display a wide variety of toxic actions. In this study, we developed a multiple-endpoints gene alteration-based (MEGA) assay, a real-time PCR-based transcriptomic analysis, to assess the water quality of wastewater effluents for human health risk assessment and management. Twenty-one genes from the human hepatoblastoma cell line (HepG2), covering the basic health-relevant stress responses such as response to xenobiotics, genotoxicity, and cytotoxicity, were selected and incorporated into the MEGA assay. The genes related to the p53-mediated DNA damage response and cytochrome P450 were selected as markers for genotoxicity and response to xenobiotics, respectively. Additionally, the genes that were dose-dependently regulated by exposure to the wastewater effluents were chosen as markers for cytotoxicity. The alterations in the expression of an individual gene, induced by exposure to the wastewater effluents, were evaluated by real-time PCR and the results were validated by genotoxicity (e.g., comet assay) and cell-based cytotoxicity tests. In summary, the MEGA assay is a real-time PCR-based assay that targets cellular responses to contaminants present in wastewater effluents at the transcriptional level; it is rapid, cost-effective, and high-throughput and can thus complement any chemical analysis for water quality assessment and management. |
| 巻・号 | 188 |
| ページ | 312-319 |
| 公開日 | 2017-12-1 |
| DOI | 10.1016/j.chemosphere.2017.08.107 |
| PII | S0045-6535(17)31330-9 |
| PMID | 28888119 |
| MeSH | Cytochrome P-450 Enzyme System / genetics DNA Damage / genetics Endpoint Determination / methods* Hep G2 Cells Humans Real-Time Polymerase Chain Reaction Toxicogenetics / methods* Wastewater / analysis* Water Pollutants, Chemical / analysis* Water Quality / standards* Xenobiotics / analysis |
| IF | 5.778 |
| 引用数 | 1 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 各媒体での言及数の合計 | 0 |
| リソース情報 | |
| ヒト・動物細胞 | Hep G2(RCB1886) |