RRC ID 52367
Author Shimizu T, Fuchimoto Y, Fukuda K, Okita H, Kitagawa Y, Kuroda T.
Title The effect of immune checkpoint inhibitors on lung metastases of osteosarcoma.
Journal J. Pediatr. Surg.
Abstract BACKGROUND/PURPOSE:The prognosis of patients with metastases remains unsatisfactory in certain pediatric solid tumors. In this study, we evaluated the efficacy of immune checkpoint inhibitors against such metastases using a murine model of osteosarcoma.
METHODS:Murine osteosarcoma LM8 cells were transplanted subcutaneously into C3H mice. The primary tumor lesion was surgically resected 11 days after transplantation. Two hundred micrograms of three antibodies (anti-PD-1, anti-PD-L1, and anti-OX-86) or an isotype antibody were administered intraperitoneally on post-transplantation days 11, 14, 18, and 21. Survival curves were plotted by the Kaplan-Meier method and compared with the log-rank test. Computed tomography (CT) scans were performed on day 11 after tumor transplantation (pre-therapy) and on day 25 (post-therapy). For pathology, 3 mice from each group were euthanized on days 11, 22, and 33 after tumor transplantation.
RESULTS:The antibody-treated group had a significantly longer survival time compared with the control group (p = 0.002). Both the CT scan and pathological results revealed suppression of metastatic tumor proliferation in the treatment group as compared with the control group.
CONCLUSIONS:These results suggest that immune checkpoint inhibitors may be an innovative therapy for lung metastases of advanced pediatric solid tumors.
Volume 52(12)
Pages 2047-2050
Published 2017-12
DOI 10.1016/j.jpedsurg.2017.08.030
PII S0022-3468(17)30527-4
PMID 28954696
MeSH Animals Antibodies, Monoclonal / therapeutic use* Antineoplastic Combined Chemotherapy Protocols / therapeutic use* B7-H1 Antigen / antagonists & inhibitors Disease Models, Animal Immunohistochemistry Immunotherapy* Kaplan-Meier Estimate Lung Neoplasms / drug therapy Lung Neoplasms / pathology Lung Neoplasms / secondary* Male Membrane Glycoproteins / antagonists & inhibitors Mice Mice, Inbred C3H Neoplasm Transplantation Osteosarcoma / drug therapy Osteosarcoma / pathology Osteosarcoma / secondary* Prognosis Programmed Cell Death 1 Receptor / antagonists & inhibitors Tomography, X-Ray Computed Tumor Necrosis Factors / antagonists & inhibitors
IF 2.128
Resource
Human and Animal Cells LM8(RCB1450)