Reference - Detail
|Author||Shimizu T, Fuchimoto Y, Fukuda K, Okita H, Kitagawa Y, Kuroda T.|
|Title||The effect of immune checkpoint inhibitors on lung metastases of osteosarcoma.|
|Journal||J. Pediatr. Surg.|
BACKGROUND/PURPOSE:The prognosis of patients with metastases remains unsatisfactory in certain pediatric solid tumors. In this study, we evaluated the efficacy of immune checkpoint inhibitors against such metastases using a murine model of osteosarcoma.
METHODS:Murine osteosarcoma LM8 cells were transplanted subcutaneously into C3H mice. The primary tumor lesion was surgically resected 11 days after transplantation. Two hundred micrograms of three antibodies (anti-PD-1, anti-PD-L1, and anti-OX-86) or an isotype antibody were administered intraperitoneally on post-transplantation days 11, 14, 18, and 21. Survival curves were plotted by the Kaplan-Meier method and compared with the log-rank test. Computed tomography (CT) scans were performed on day 11 after tumor transplantation (pre-therapy) and on day 25 (post-therapy). For pathology, 3 mice from each group were euthanized on days 11, 22, and 33 after tumor transplantation.
RESULTS:The antibody-treated group had a significantly longer survival time compared with the control group (p = 0.002). Both the CT scan and pathological results revealed suppression of metastatic tumor proliferation in the treatment group as compared with the control group.
CONCLUSIONS:These results suggest that immune checkpoint inhibitors may be an innovative therapy for lung metastases of advanced pediatric solid tumors.
|MeSH||Animals Antibodies, Monoclonal / therapeutic use* Antineoplastic Combined Chemotherapy Protocols / therapeutic use* B7-H1 Antigen / antagonists & inhibitors Disease Models, Animal Immunohistochemistry Immunotherapy* Kaplan-Meier Estimate Lung Neoplasms / drug therapy Lung Neoplasms / pathology Lung Neoplasms / secondary* Male Membrane Glycoproteins / antagonists & inhibitors Mice Mice, Inbred C3H Neoplasm Transplantation Osteosarcoma / drug therapy Osteosarcoma / pathology Osteosarcoma / secondary* Prognosis Programmed Cell Death 1 Receptor / antagonists & inhibitors Tomography, X-Ray Computed Tumor Necrosis Factors / antagonists & inhibitors|
|Human and Animal Cells||LM8(RCB1450)|