論文 - 詳細
| RRC ID | 52367 |
|---|---|
| 著者 | Shimizu T, Fuchimoto Y, Fukuda K, Okita H, Kitagawa Y, Kuroda T. |
| タイトル | The effect of immune checkpoint inhibitors on lung metastases of osteosarcoma. |
| ジャーナル | J Pediatr Surg |
| Abstract |
BACKGROUND/PURPOSE:The prognosis of patients with metastases remains unsatisfactory in certain pediatric solid tumors. In this study, we evaluated the efficacy of immune checkpoint inhibitors against such metastases using a murine model of osteosarcoma. METHODS:Murine osteosarcoma LM8 cells were transplanted subcutaneously into C3H mice. The primary tumor lesion was surgically resected 11 days after transplantation. Two hundred micrograms of three antibodies (anti-PD-1, anti-PD-L1, and anti-OX-86) or an isotype antibody were administered intraperitoneally on post-transplantation days 11, 14, 18, and 21. Survival curves were plotted by the Kaplan-Meier method and compared with the log-rank test. Computed tomography (CT) scans were performed on day 11 after tumor transplantation (pre-therapy) and on day 25 (post-therapy). For pathology, 3 mice from each group were euthanized on days 11, 22, and 33 after tumor transplantation. RESULTS:The antibody-treated group had a significantly longer survival time compared with the control group (p = 0.002). Both the CT scan and pathological results revealed suppression of metastatic tumor proliferation in the treatment group as compared with the control group. CONCLUSIONS:These results suggest that immune checkpoint inhibitors may be an innovative therapy for lung metastases of advanced pediatric solid tumors. |
| 巻・号 | 52(12) |
| ページ | 2047-2050 |
| 公開日 | 2017-12-1 |
| DOI | 10.1016/j.jpedsurg.2017.08.030 |
| PII | S0022-3468(17)30527-4 |
| PMID | 28954696 |
| MeSH | Animals Antibodies, Monoclonal / therapeutic use* Antineoplastic Combined Chemotherapy Protocols / therapeutic use* B7-H1 Antigen / antagonists & inhibitors Disease Models, Animal Immunohistochemistry Immunotherapy* Kaplan-Meier Estimate Lung Neoplasms / drug therapy Lung Neoplasms / pathology Lung Neoplasms / secondary* Male Membrane Glycoproteins / antagonists & inhibitors Mice Mice, Inbred C3H Neoplasm Transplantation OX40 Ligand Osteosarcoma / drug therapy Osteosarcoma / pathology Osteosarcoma / secondary* Prognosis Programmed Cell Death 1 Receptor / antagonists & inhibitors Tomography, X-Ray Computed Tumor Necrosis Factor Inhibitors |
| IF | 2.128 |
| 引用数 | 7 |
| リソース情報 | |
| ヒト・動物細胞 | LM8(RCB1450) |