| RRC ID |
52378
|
| Author |
Okutani C, Wagatsuma A, Mabuchi K, Hoshino T.
|
| Title |
Directed cell migration in co-cultures by topographic curvature for heterogeneous tissue engineering.
|
| Journal |
Annu Int Conf IEEE Eng Med Biol Soc
|
| Abstract |
Placing cells in the proper position is important for tissue engineering. Previous works addressed this subject in the way of controlling cell migration by micro- or nano-patterning the substrates. However, the problem of changing spatial cell density freely under co-culture conditions is remaining. To solve this problem, in this work, we report that C2C12 spatial cell density changes by the patterning geometric boundary of the topographical structures. In 48 h after seeding cells, at the linear boundary (ridge-groove) structures, C2C12 Groove/Ridge ratio was under 0.70 both under monoculture conditions and under co-culture conditions. In contrast, at the combining the linear boundary and the round boundary (ridge-groove + hole) structures, the ratio was over 0.89 under both culture conditions. This our finding will provide a new device which enables to manipulate spatial cell density under co-culture conditions for heterogeneous tissue engineering.
|
| Volume |
2017
|
| Pages |
1607-1610
|
| Published |
2017-7-1
|
| DOI |
10.1109/EMBC.2017.8037146
|
| PMID |
29060190
|
| MeSH |
Cell Count
Cell Culture Techniques
Cell Movement
Coculture Techniques
Tissue Engineering*
|
| Times Cited |
1
|
| Resource |
| Human and Animal Cells |
C2C12(RCB0987)
PC-12(RCB0009) |
