論文 - 詳細
| RRC ID | 52386 |
|---|---|
| 著者 | Zhou Y, Fukuda T, Hang Q, Hou S, Isaji T, Kameyama A, Gu J. |
| タイトル | Inhibition of fucosylation by 2-fluorofucose suppresses human liver cancer HepG2 cell proliferation and migration as well as tumor formation. |
| ジャーナル | Sci Rep |
| Abstract |
Core fucosylation is one of the most important glycosylation events in the progression of liver cancer. For this study, we used an easily handled L-fucose analog, 2-fluoro-L-fucose (2FF), which interferes with the normal synthesis of GDP-fucose, and verified its potential roles in regulating core fucosylation and cell behavior in the HepG2 liver cancer cell line. Results obtained from lectin blot and flow cytometry analysis clearly showed that 2FF treatment dramatically inhibited core fucosylation, which was also confirmed via mass spectrometry analysis. Cell proliferation and integrin-mediated cell migration were significantly suppressed in cells treated with 2FF. We further analyzed cell colony formation in soft agar and tumor xenograft efficacy, and found that both were greatly suppressed in the 2FF-treated cells, compared with the control cells. Moreover, the treatment with 2FF decreased the core fucosylation levels of membrane glycoproteins such as EGF receptor and integrin β1, which in turn suppressed downstream signals that included phospho-EGFR, -AKT, -ERK, and -FAK. These results clearly described the roles of 2FF and the importance of core fucosylation in liver cancer progression, suggesting 2FF shows promise for use in the treatment of hepatoma. |
| 巻・号 | 7(1) |
| ページ | 11563 |
| 公開日 | 2017-9-14 |
| DOI | 10.1038/s41598-017-11911-9 |
| PII | 10.1038/s41598-017-11911-9 |
| PMID | 28912543 |
| PMC | PMC5599613 |
| MeSH | Cell Line, Tumor Cell Movement / drug effects* Cell Proliferation / drug effects Cell Transformation, Neoplastic / drug effects* ErbB Receptors / metabolism Fucose / pharmacology* Glycosylation Hep G2 Cells Humans Integrins / metabolism Intracellular Space / metabolism Polysaccharides / metabolism Signal Transduction / drug effects |
| IF | 3.998 |
| 引用数 | 17 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 2 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | Hep G2 HeLa |