| RRC ID |
52395
|
| 著者 |
Liu Y, Wu Z, Nakanishi Y, Ni J, Hayashi Y, Takayama F, Zhou Y, Kadowaki T, Nakanishi H.
|
| タイトル |
Infection of microglia with Porphyromonas gingivalis promotes cell migration and an inflammatory response through the gingipain-mediated activation of protease-activated receptor-2 in mice.
|
| ジャーナル |
Sci Rep
|
| Abstract |
Despite a clear correlation between periodontitis and cognitive decline in Alzheimer's disease, the precise mechanism underlying the relationship remains unclear. The periodontal pathogen Porphyromonas gingivalis produces a unique class of cysteine proteinases termed gingipains that comprises Arg-gingipain (Rgp) and Lys-gingipain (Kgp). Rgp and Kgp are important in the bacterial mediated host cell responses and the subsequent intracellular signaling in infected cells. In the present study, we attempted to clarify the potential effects of Rgp and Kgp on the cellular activation of brain-resident microglia. We provide the first evidence that Rgp and Kgp cooperatively contribute to the P. gingivalis-induced cell migration and expression of proinflammatory mediators through the activation of protease-activated receptor 2. The subsequent activation of phosphoinositide 3-kinase/Akt and mitogen-activated protein kinase/extracellular signal-regulated kinase (ERK) kinase/ERK pathways contributes to cell migration and inflammatory response of microglia.
|
| 巻・号 |
7(1)
|
| ページ |
11759
|
| 公開日 |
2017-9-18
|
| DOI |
10.1038/s41598-017-12173-1
|
| PII |
10.1038/s41598-017-12173-1
|
| PMID |
28924232
|
| PMC |
PMC5603557
|
| MeSH |
Animals
Bacteroidaceae Infections / genetics
Bacteroidaceae Infections / metabolism*
Bacteroidaceae Infections / pathology
Cell Movement*
Cysteine Endopeptidases / metabolism*
Extracellular Signal-Regulated MAP Kinases
Gingipain Cysteine Endopeptidases
MAP Kinase Signaling System*
Mice
Mice, Transgenic
Microglia / metabolism*
Microglia / microbiology
Phosphatidylinositol 3-Kinases
Porphyromonas gingivalis / metabolism*
Porphyromonas gingivalis / pathogenicity
Proto-Oncogene Proteins c-akt
Receptor, PAR-2 / genetics
Receptor, PAR-2 / metabolism*
|
| IF |
3.998
|
| 引用数 |
4
|
| リソース情報 |
| ヒト・動物細胞 |
MG6(RCB2403) |
