| RRC ID |
524
|
| 著者 |
Reza AM, Kanamori Y, Shinoda T, Shimura S, Mita K, Nakahara Y, Kiuchi M, Kamimura M.
|
| タイトル |
Hormonal control of a metamorphosis-specific transcriptional factor Broad-Complex in silkworm.
|
| ジャーナル |
Comp Biochem Physiol B Biochem Mol Biol
|
| Abstract |
Insect metamorphosis is induced by the steroid 20-hydroxyecdysone (20E) in the absence of sesquiterpenoid juvenile hormone (JH). In Drosophila melanogaster, the Broad-Complex (BR-C) transcriptional factor plays critical roles during metamorphosis. We isolated cDNAs encoding BR-C in the silkworm Bombyx mori and examined their mRNA expression. cDNAs for three BR-C isoforms with zinc finger pairs (Z1, Z2 and Z4) and four isoforms lacking them were cloned. Their mRNAs were expressed in multiple tissues at the larval-pupal metamorphosis. In the anterior silk gland, BR-C mRNAs were expressed at the end of the last larval instar but not expressed during the penultimate instar. 20E administration induced BR-C mRNA expression and JH suppressed 20E-induced BR-C expression in this tissue both in vivo and in vitro. Thus, BR-C mRNAs are inducible by 20E only in the absence of JH, a finding that explains their metamorphosis-specific expression.
|
| 巻・号 |
139(4)
|
| ページ |
753-61
|
| 公開日 |
2004-12-1
|
| DOI |
10.1016/j.cbpc.2004.09.009
|
| PII |
S1096-4959(04)00282-9
|
| PMID |
15581808
|
| MeSH |
Animals
Bombyx / genetics
Bombyx / physiology*
Drosophila melanogaster / genetics
Ecdysterone / genetics
Ecdysterone / metabolism*
Gene Expression Regulation, Developmental / genetics
Gene Expression Regulation, Developmental / physiology*
Juvenile Hormones / genetics
Juvenile Hormones / metabolism*
Larva / genetics
Larva / physiology
Metamorphosis, Biological / genetics
Metamorphosis, Biological / physiology*
RNA, Messenger / genetics
Transcription Factors / genetics
Transcription Factors / metabolism*
|
| IF |
2.219
|
| 引用数 |
53
|
|
WOS 分野
|
ZOOLOGY
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| リソース情報 |
| カイコ |
|
