論文 - 詳細
| RRC ID | 52453 |
|---|---|
| 著者 | Takata N, Sakakura E, Eiraku M, Kasukawa T, Sasai Y. |
| タイトル | Self-patterning of rostral-caudal neuroectoderm requires dual role of Fgf signaling for localized Wnt antagonism. |
| ジャーナル | Nat Commun |
| Abstract |
The neuroectoderm is patterned along a rostral-caudal axis in response to localized factors in the embryo, but exactly how these factors act as positional information for this patterning is not yet fully understood. Here, using the self-organizing properties of mouse embryonic stem cell (ESC), we report that ESC-derived neuroectoderm self-generates a Six3+ rostral and a Irx3+ caudal bipolarized patterning. In this instance, localized Fgf signaling performs dual roles, as it regulates Six3+ rostral polarization at an earlier stage and promotes Wnt signaling at a later stage. The Wnt signaling components are differentially expressed in the polarized tissues, leading to genome-wide Irx3+ caudal-polarization signals. Surprisingly, differentially expressed Wnt agonists and antagonists have essential roles in orchestrating the formation of a balanced rostral-caudal neuroectoderm pattern. Together, our findings provide key processes for dynamic self-patterning and evidence that a temporally and locally regulated interaction between Fgf and Wnt signaling controls self-patterning in ESC-derived neuroectoderm. |
| 巻・号 | 8(1) |
| ページ | 1339 |
| 公開日 | 2017-11-7 |
| DOI | 10.1038/s41467-017-01105-2 |
| PII | 10.1038/s41467-017-01105-2 |
| PMID | 29109536 |
| PMC | PMC5673904 |
| MeSH | Animals Body Patterning Embryonic Stem Cells Eye Proteins / genetics Eye Proteins / metabolism Fibroblast Growth Factor 5 / genetics Fibroblast Growth Factor 5 / metabolism Fibroblast Growth Factors / genetics Fibroblast Growth Factors / metabolism* Gene Expression Regulation, Developmental Homeodomain Proteins / genetics Homeodomain Proteins / metabolism Intercellular Signaling Peptides and Proteins / genetics Intercellular Signaling Peptides and Proteins / metabolism Membrane Proteins / genetics Membrane Proteins / metabolism Mice, Inbred ICR Mice, Transgenic Nerve Tissue Proteins / genetics Nerve Tissue Proteins / metabolism Neural Plate / metabolism* Neural Plate / physiology Signal Transduction Transcription Factors / genetics Transcription Factors / metabolism Wnt Proteins / genetics Wnt Proteins / metabolism* |
| IF | 12.121 |
| 引用数 | 12 |
| リソース情報 | |
| ヒト・動物細胞 | EB5(AES0151) |