RRC ID 52483
Author Kikuchi H, Mimuro H, Kuribayashi F.
Title Resveratrol strongly enhances the retinoic acid-induced superoxide generating activity via up-regulation of gp91-phox gene expression in U937 cells.
Journal Biochem Biophys Res Commun
Abstract The membrane bound cytochrome b558 composed of gp91-phox and p22-phox proteins, and cytosolic proteins p40-, p47-and p67-phox are important components of superoxide (O2-)-generating system in phagocytes. Here, we describe that resveratrol, a pleiotropic phytochemical belonging to the stilbenoids, dramatically activates the O2--generating system during retinoic acid (RA)-induced differentiation of human monoblastic leukemia U937 cells to macrophage-like cells. When U937 cells were cultured in the presence of RA and resveratrol, the O2--generating activity increased more than 5-fold compared with that in the absence of the latter. Semiquantitative RT-PCR showed that co-treatment with RA and resveratrol strongly enhanced transcription of the gp91-phox compared with those of the RA-treatment only. On the other hand, immunoblot analysis revealed that co-treatment with RA and resveratrol caused remarkable accumulation of protein levels of gp91-phox (to 4-fold), p22-phox (to 5-fold) and p47-phox (to 4-fold) compared with those of the RA-treatment alone. In addition, ChIP assay suggested that resveratrol participates in enhancing the gene expression of gp91-phox via promoting acetylation of Lys-9 residues and Lys-14 residues of histone H3 within chromatin around the promoter regions of the gene. These results suggested that resveratrol strongly enhances the RA-induced O2--generating activity via up-regulation of gp91-phox gene expression in U937 cells.
Volume 495(1)
Pages 1195-1200
Published 2018-1-1
DOI 10.1016/j.bbrc.2017.11.161
PII S0006-291X(17)32338-0
PMID 29183727
MeSH Dose-Response Relationship, Drug Gene Expression Regulation, Neoplastic / drug effects* Humans NADPH Oxidase 2 / metabolism* Neoplasms, Experimental / metabolism* Resveratrol Stilbenes / administration & dosage* Superoxides / metabolism* Tretinoin / metabolism* U937 Cells Up-Regulation / drug effects
IF 2.985
Times Cited 4
Human and Animal Cells U-937 DE-4(RCB0435)