論文 - 詳細
| RRC ID | 52500 |
|---|---|
| 著者 | Mikami R, Mizutani K, Aoki A, Tamura Y, Aoki K, Izumi Y. |
| タイトル | Low-level ultrahigh-frequency and ultrashort-pulse blue laser irradiation enhances osteoblast extracellular calcification by upregulating proliferation and differentiation via transient receptor potential vanilloid 1. |
| ジャーナル | Lasers Surg Med |
| Abstract |
BACKGROUND AND OBJECTIVE:Low-level laser irradiation (LLLI) exerts various biostimulative effects, including promotion of wound healing and bone formation; however, few studies have examined biostimulation using blue lasers. The purpose of this study was to investigate the effects of low-level ultrahigh-frequency (UHF) and ultrashort-pulse (USP) blue laser irradiation on osteoblasts. STUDY DESIGN/ MATERIALS AND METHODS:The MC3T3-E1 osteoblast cell line was used in this study. Following LLLI with a 405 nm newly developed UHF-USP blue laser (80 MHz, 100 fs), osteoblast proliferation, and alkaline phosphatase (ALP) activity were assessed. In addition, mRNA levels of the osteoblast differentiation markers, runt-related transcription factor 2 (Runx2), osterix (Osx), alkaline phosphatase (Alp), and osteopontin (Opn) was evaluated, and extracellular calcification was quantified. To clarify the involvement of transient receptor potential (TRP) channels in LLLI-induced biostimulation, cells were treated prior to LLLI with capsazepine (CPZ), a selective inhibitor of TRP vanilloid 1 (TRPV1), and subsequent proliferation and ALP activity were measured. RESULTS:LLLI with the 405 nm UHF-USP blue laser significantly enhanced cell proliferation and ALP activity, compared with the non-irradiated control and LLLI using continuous-wave mode, without significant temperature elevation. LLLI promoted osteoblast proliferation in a dose-dependent manner up to 9.4 J/cm2 and significantly accelerated cell proliferation in in vitro wound healing assay. ALP activity was significantly enhanced at doses up to 5.6 J/cm2 , and expression of Osx and Alp mRNAs was significantly increased compared to that of the control on days 3 and 7 following LLLI at 5.6 J/cm2 . The extent of extracellular calcification was also significantly higher as a result of LLLI 3 weeks after the treatment. Measurement of TRPV1 protein expression on 0, 3, and 7 days post-irradiation revealed no differences between the LLLI and control groups; however, promotion of cell proliferation and ALP activity by LLLI was significantly inhibited by CPZ. CONCLUSION:LLLI with a 405 nm UHF-USP blue laser enhances extracellular calcification of osteoblasts by upregulating proliferation and differentiation via TRPV1. Lasers Surg. Med. 50:340-352, 2018. © 2017 Wiley Periodicals, Inc. |
| 巻・号 | 50(4) |
| ページ | 340-352 |
| 公開日 | 2018-4-1 |
| DOI | 10.1002/lsm.22775 |
| PMID | 29214666 |
| MeSH | Alkaline Phosphatase / metabolism Animals Calcinosis / physiopathology Cell Line / radiation effects Cell Proliferation / radiation effects Lasers, Dye / therapeutic use Low-Level Light Therapy / methods* Mice Osteoblasts / physiology* Osteoblasts / radiation effects* Osteopontin / metabolism Osteopontin / radiation effects Real-Time Polymerase Chain Reaction / methods Sensitivity and Specificity TRPV Cation Channels / genetics* TRPV Cation Channels / radiation effects* Up-Regulation |
| IF | 3.02 |
| 引用数 | 8 |
| リソース情報 | |
| ヒト・動物細胞 | MC3T3-E1(RCB1126) |