RRC ID 52502
Author Nagahara H, Seno T, Yamamoto A, Obayashi H, Inoue T, Kida T, Nakabayashi A, Kukida Y, Fujioka K, Fujii W, Murakami K, Kohno M, Kawahito Y.
Title Role of allograft inflammatory factor-1 in bleomycin-induced lung fibrosis.
Journal Biochem Biophys Res Commun
Abstract Allograft inflammatory factor-1 (AIF-1) is a protein expressed by macrophages infiltrating the area around the coronary arteries in a rat ectopic cardiac allograft model. We previously reported that AIF-1 is associated with the pathogenesis of rheumatoid arthritis and skin fibrosis in sclerodermatous graft-versus-host disease mice. Here, we used an animal model of bleomycin-induced lung fibrosis to analyze the expression of AIF-1 and examine its function in lung fibrosis. The results showed that AIF-1 was expressed on lung tissues, specifically macrophages, from mice with bleomycin-induced lung fibrosis. Recombinant AIF-1 increased the production of TGF-β which plays crucial roles in the mechanism of fibrosis by mouse macrophage cell line RAW264.7. Recombinant AIF-1 also increased both the proliferation and migration of lung fibroblasts compared with control group. These results suggest that AIF-1 plays an important role in the mechanism underlying lung fibrosis, and may provide an attractive new therapeutic target.
Volume 495(2)
Pages 1901-1907
Published 2018-1-8
DOI 10.1016/j.bbrc.2017.12.035
PII S0006-291X(17)32427-0
PMID 29225172
MeSH Animals Bleomycin* Calcium-Binding Proteins / immunology* Cells, Cultured Immunologic Factors / immunology* Macrophage Activation / immunology* Macrophages / immunology* Macrophages / pathology Mice Mice, Inbred C57BL Microfilament Proteins / immunology* Pulmonary Fibrosis / chemically induced* Pulmonary Fibrosis / immunology* Pulmonary Fibrosis / pathology
IF 2.985
Times Cited 3
Human and Animal Cells RAW 264(RCB0535)