Reference - Detail
|Author||Nagahara H, Seno T, Yamamoto A, Obayashi H, Inoue T, Kida T, Nakabayashi A, Kukida Y, Fujioka K, Fujii W, Murakami K, Kohno M, Kawahito Y.|
|Title||Role of allograft inflammatory factor-1 in bleomycin-induced lung fibrosis.|
|Journal||Biochem Biophys Res Commun|
Allograft inflammatory factor-1 (AIF-1) is a protein expressed by macrophages infiltrating the area around the coronary arteries in a rat ectopic cardiac allograft model. We previously reported that AIF-1 is associated with the pathogenesis of rheumatoid arthritis and skin fibrosis in sclerodermatous graft-versus-host disease mice. Here, we used an animal model of bleomycin-induced lung fibrosis to analyze the expression of AIF-1 and examine its function in lung fibrosis. The results showed that AIF-1 was expressed on lung tissues, specifically macrophages, from mice with bleomycin-induced lung fibrosis. Recombinant AIF-1 increased the production of TGF-β which plays crucial roles in the mechanism of fibrosis by mouse macrophage cell line RAW264.7. Recombinant AIF-1 also increased both the proliferation and migration of lung fibroblasts compared with control group. These results suggest that AIF-1 plays an important role in the mechanism underlying lung fibrosis, and may provide an attractive new therapeutic target.
|MeSH||Animals Bleomycin* Calcium-Binding Proteins / immunology* Cells, Cultured Immunologic Factors / immunology* Macrophage Activation / immunology* Macrophages / immunology* Macrophages / pathology Mice Mice, Inbred C57BL Microfilament Proteins / immunology* Pulmonary Fibrosis / chemically induced* Pulmonary Fibrosis / immunology* Pulmonary Fibrosis / pathology|
|Human and Animal Cells||RAW 264(RCB0535)|