RRC ID 52504
Author Ono-Ohmachi A, Ishida Y, Morita Y, Kato K, Y Nara T.
Title Milk Basic Protein Facilitates Increased Bone Mass in Growing Mice.
Journal J Nutr Sci Vitaminol (Tokyo)
Abstract Milk basic protein (MBP) comprises a group of basic whey proteins and is effective in preventing bone loss by promoting bone deposition (bone formation) and suppressing withdrawn (bone resorption). We previously revealed the bone protective effects of MBP during life phases involving excessive bone resorption, such as in adults and postmenopausal women, and in animal models (ovariectomized rats and mice). However, it was unclear whether MBP increases bone mass during the growth stage, when there is more bone formation than resorption. We therefore investigated the effect of MBP supplementation on bone mass in 6-wk-old mice provided water supplemented with MBP [0.01%, 0.1%, 1.0% (w/w)] or deionized water (control) ad libitum for 10 wk. Analysis by micro-computerized tomography showed that MBP significantly increased tibia cortical bone mineral density and femur trabecular bone volume to tissue volume compared with mice provided deionized water. Next, the function of MBP in bone remodeling (bone formation and resorption) was evaluated using an in vitro system and the results demonstrated that MBP directly promoted osteoblast proliferation and inhibited osteoclastogenesis. Moreover, the plasma level of insulin-like growth factor-1 was increased by MBP supplementation, suggesting that MBP indirectly promoted osteoblast proliferation/differentiation. These effects enhance bone formation and/or inhibit bone resorption, resulting in increased bone mass in growing mice.
Volume 63(5)
Pages 315-322
Published 2017-1-1
DOI 10.3177/jnsv.63.315
PMID 29225316
MeSH Animals Biomarkers / blood Biomarkers / metabolism Bone Density Bone Density Conservation Agents / administration & dosage Bone Density Conservation Agents / metabolism Bone Density Conservation Agents / therapeutic use Bone Remodeling Bone Resorption / blood Bone Resorption / metabolism Bone Resorption / pathology Bone Resorption / prevention & control Cancellous Bone / cytology Cancellous Bone / diagnostic imaging Cancellous Bone / growth & development* Cancellous Bone / pathology Cell Proliferation Cells, Cultured Cortical Bone / cytology Cortical Bone / diagnostic imaging Cortical Bone / growth & development* Cortical Bone / pathology Dietary Supplements* Insulin-Like Growth Factor I / metabolism Macrophages / cytology Macrophages / metabolism Macrophages / pathology Male Mice Milk Proteins / administration & dosage* Milk Proteins / metabolism Milk Proteins / therapeutic use Osteoblasts / cytology* Osteoblasts / metabolism Osteoblasts / pathology Osteoclasts / cytology* Osteoclasts / metabolism Osteoclasts / pathology Osteogenesis* Tomography Scanners, X-Ray Computed
IF 1.125
Times Cited 1
Human and Animal Cells ST2(RCB0224)