| RRC ID |
52626
|
| 著者 |
Morio H, Sun Y, Harada M, Ide H, Shimozato O, Zhou X, Higashi K, Yuki R, Yamaguchi N, Hofbauer JP, Guttmann-Gruber C, Anzai N, Akita H, Chiba K, Furihata T.
|
| タイトル |
Cancer-Type OATP1B3 mRNA in Extracellular Vesicles as a Promising Candidate for a Serum-Based Colorectal Cancer Biomarker.
|
| ジャーナル |
Biol Pharm Bull
|
| Abstract |
Cancer-type organic anion transporting polypeptide 1B3 (Ct-OATP1B3) mRNA is a variant isoform of the liver-type OATP1B3. Because Ct-OATP1B3 mRNA shows an excellent cancer-specific expression profile in colorectal cancer (CRC), and that its expression levels are associated with CRC prognosis, it holds the potential to become a useful CRC detection and diagnosis biomarker. While the potential is currently justified only at the tissue level, if existence of Ct-OATP1B3 mRNA in CRC-derived extracellular vesicles (EVs) is validated, the findings could enhance its translational potential as a CRC detection and diagnosis biomarker. Therefore, this study aims at proving that Ct-OATP1B3 mRNA exists in CRC-derived EVs, and can be detected using serum specimens. To examine the possibility of Ct-OATP1B3 mRNA being existed in extracellular milieu, we isolated EVs from the human CRC (HCT116, HT-29, and SW480) cell lines, and prepared their cDNAs. The RT-PCR results showed that Ct-OATP1B3 mRNA was clearly present in EVs derived from the human CRC cell lines. Then, in order to further explore the possibility that Ct-OATP1B3 mRNA in CRC-derived EVs can be detected in serum, we isolated serum EVs derived from human CRC xenograft mice, and then performed RT-PCR. The results showed that Ct-OATP1B3 mRNA could be found in all serum EV and CRC tissue samples of the mice examined. Collectively, our findings, which show that Ct-OATP1B3 mRNA exists in EVs and can be detected in (at least) mouse serum, strengthen the potential use of Ct-OATP1B3 mRNA as a serum-based CRC biomarker.
|
| 巻・号 |
41(3)
|
| ページ |
445-449
|
| 公開日 |
2018-1-1
|
| DOI |
10.1248/bpb.b17-00743
|
| PMID |
29491222
|
| MeSH |
Animals
Biomarkers, Tumor / genetics*
Cell Line, Tumor
Colorectal Neoplasms / blood*
Colorectal Neoplasms / diagnosis
Extracellular Vesicles / metabolism*
Gene Expression Regulation, Neoplastic
Humans
Male
Mice
Mice, Inbred BALB C
Neoplasm Transplantation
Prognosis
RNA, Messenger / blood*
RNA, Messenger / genetics*
RNA, Neoplasm / blood*
RNA, Neoplasm / genetics*
Real-Time Polymerase Chain Reaction
Solute Carrier Organic Anion Transporter Family Member 1B3 / biosynthesis
Solute Carrier Organic Anion Transporter Family Member 1B3 / genetics*
|
| IF |
1.863
|
| 引用数 |
4
|
| リソース情報 |
| ヒト・動物細胞 |
Colon-26(RCB2657) |
