論文 - 詳細
| RRC ID | 52631 |
|---|---|
| 著者 | Saito S, Hiemori K, Kiyoi K, Tateno H. |
| タイトル | Glycome analysis of extracellular vesicles derived from human induced pluripotent stem cells using lectin microarray. |
| ジャーナル | Sci Rep |
| Abstract |
Glycans are one of the major building blocks of extracellular vesicles (EVs). However, their roles and applications have not been completely explored. Here, we analyzed the glycome of EVs derived from human induced pluripotent stem cells (hiPSCs) using high-density lectin microarray. The glycan profiles of hiPSC-derived EVs were different from those of non-hiPSC-derived EVs. Moreover, rBC2LCN that shows specific binding to hiPSCs, showed strong specificity for hiPSC-derived EVs but not non-hiPSCs-derived EVs. Further, other hiPSC-specific probes, such as anti-TRA-1-60, anti-SSEA4, and anti-R-10G, exhibited specific, but weaker binding to hiPSC-derived EVs than rBC2LCN. We then developed a sandwich assay using rBC2LCN and a phosphatidylserine receptor, Tim4, to specifically detect hiPSC-derived EVs. The Tim4-rBC2LCN sandwich assay allowed for specific detection of hiPSC-derived EVs but not non-hiPSC-derived EVs, indicating that rBC2LCN could also be used for the specific detection of hiPSC-derived EVs. Together, our findings demonstrate that the characteristic glycan signature of hiPSCs are retained by EVs derived from them. The EV glycome could be novel targets for the identification and characterization of stem cells for use in regenerative medicine. |
| 巻・号 | 8(1) |
| ページ | 3997 |
| 公開日 | 2018-3-5 |
| DOI | 10.1038/s41598-018-22450-2 |
| PII | 10.1038/s41598-018-22450-2 |
| PMID | 29507392 |
| PMC | PMC5838221 |
| MeSH | Cell Differentiation Cells, Cultured Culture Media Extracellular Vesicles / metabolism* Flow Cytometry Humans Induced Pluripotent Stem Cells / cytology Induced Pluripotent Stem Cells / metabolism* Lectins / metabolism* Ligands Polysaccharides / metabolism* Quality Control Regenerative Medicine Sialoglycoproteins / metabolism |
| IF | 3.998 |
| 引用数 | 11 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 3 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | 201B7(HPS0063) Yub622(HMS0016) Yub625(HMS0038) |