論文 - 詳細
| RRC ID | 52719 |
|---|---|
| 著者 | Sato K, Yamashita T, Ohuchi H, Takeuchi A, Gotoh H, Ono K, Mizuno M, Mizutani Y, Tomonari S, Sakai K, Imamoto Y, Wada A, Shichida Y. |
| タイトル | Opn5L1 is a retinal receptor that behaves as a reverse and self-regenerating photoreceptor. |
| ジャーナル | Nat Commun |
| Abstract |
Most opsins are G protein-coupled receptors that utilize retinal both as a ligand and as a chromophore. Opsins' main established mechanism is light-triggered activation through retinal 11-cis-to-all-trans photoisomerization. Here we report a vertebrate non-visual opsin that functions as a Gi-coupled retinal receptor that is deactivated by light and can thermally self-regenerate. This opsin, Opn5L1, binds exclusively to all-trans-retinal. More interestingly, the light-induced deactivation through retinal trans-to-cis isomerization is followed by formation of a covalent adduct between retinal and a nearby cysteine, which breaks the retinal-conjugated double bond system, probably at the C11 position, resulting in thermal re-isomerization to all-trans-retinal. Thus, Opn5L1 acts as a reverse photoreceptor. We conclude that, like vertebrate rhodopsin, Opn5L1 is a unidirectional optical switch optimized from an ancestral bidirectional optical switch, such as invertebrate rhodopsin, to increase the S/N ratio of the signal transduction, although the direction of optimization is opposite to that of vertebrate rhodopsin. |
| 巻・号 | 9(1) |
| ページ | 1255 |
| 公開日 | 2018-3-28 |
| DOI | 10.1038/s41467-018-03603-3 |
| PII | 10.1038/s41467-018-03603-3 |
| PMID | 29593298 |
| PMC | PMC5871776 |
| MeSH | Animals Chickens Chromatography, High Pressure Liquid Factor Xa / chemistry HEK293 Cells Humans In Situ Hybridization Light Male Opsins / chemistry* Photoreceptor Cells Photoreceptor Cells, Vertebrate / chemistry* Protein Binding Recombinant Proteins / chemistry Regeneration Retinaldehyde / metabolism Rhodopsin / chemistry Signal Transduction Vitamin A / chemistry Xenopus / metabolism |
| IF | 12.121 |
| 引用数 | 0 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 56 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 3.5 |
| リソース情報 | |
| ツメガエル・イモリ | Xenopus tropicalis |