| RRC ID |
52719
|
| 著者 |
Sato K, Yamashita T, Ohuchi H, Takeuchi A, Gotoh H, Ono K, Mizuno M, Mizutani Y, Tomonari S, Sakai K, Imamoto Y, Wada A, Shichida Y.
|
| タイトル |
Opn5L1 is a retinal receptor that behaves as a reverse and self-regenerating photoreceptor.
|
| ジャーナル |
Nat Commun
|
| Abstract |
Most opsins are G protein-coupled receptors that utilize retinal both as a ligand and as a chromophore. Opsins' main established mechanism is light-triggered activation through retinal 11-cis-to-all-trans photoisomerization. Here we report a vertebrate non-visual opsin that functions as a Gi-coupled retinal receptor that is deactivated by light and can thermally self-regenerate. This opsin, Opn5L1, binds exclusively to all-trans-retinal. More interestingly, the light-induced deactivation through retinal trans-to-cis isomerization is followed by formation of a covalent adduct between retinal and a nearby cysteine, which breaks the retinal-conjugated double bond system, probably at the C11 position, resulting in thermal re-isomerization to all-trans-retinal. Thus, Opn5L1 acts as a reverse photoreceptor. We conclude that, like vertebrate rhodopsin, Opn5L1 is a unidirectional optical switch optimized from an ancestral bidirectional optical switch, such as invertebrate rhodopsin, to increase the S/N ratio of the signal transduction, although the direction of optimization is opposite to that of vertebrate rhodopsin.
|
| 巻・号 |
9(1)
|
| ページ |
1255
|
| 公開日 |
2018-3-28
|
| DOI |
10.1038/s41467-018-03603-3
|
| PII |
10.1038/s41467-018-03603-3
|
| PMID |
29593298
|
| PMC |
PMC5871776
|
| MeSH |
Animals
Chickens
Chromatography, High Pressure Liquid
Factor Xa / chemistry
HEK293 Cells
Humans
In Situ Hybridization
Light
Male
Opsins / chemistry*
Photoreceptor Cells
Photoreceptor Cells, Vertebrate / chemistry*
Protein Binding
Recombinant Proteins / chemistry
Regeneration
Retinaldehyde / metabolism
Rhodopsin / chemistry
Signal Transduction
Vitamin A / chemistry
Xenopus / metabolism
|
| IF |
12.121
|
| 引用数 |
0
|
| リソース情報 |
| ツメガエル・イモリ |
Xenopus tropicalis |
