RRC ID 52726
Author Kusunoki K, Nakano Y, Tanaka K, Sakata Y, Koyama H, Kobayashi Y.
Title Transcriptomic variation among six Arabidopsis thaliana accessions identified several novel genes controlling aluminium tolerance.
Journal Plant Cell Environ
Abstract Differences in the expression levels of aluminium (Al) tolerance genes are a known determinant of Al tolerance among plant varieties. We combined transcriptomic analysis of six Arabidopsis thaliana accessions with contrasting Al tolerance and a reverse genetic approach to identify Al-tolerance genes responsible for differences in Al tolerance between accession groups. Gene expression variation increased in the signal transduction process under Al stress and in growth-related processes in the absence of stress. Co-expression analysis and promoter single nucleotide polymorphism searching suggested that both trans-acting polymorphisms of Al signal transduction pathway and cis-acting polymorphisms in the promoter sequences caused the variations in gene expression associated with Al tolerance. Compared with the wild type, Al sensitivity increased in T-DNA knockout (KO) lines for five genes, including TARGET OF AVRB OPERATION1 (TAO1) and an unannotated gene (At5g22530). These were identified from 53 Al-inducible genes showing significantly higher expression in tolerant accessions than in sensitive accessions. These results indicate that the difference in transcriptional signalling is partly associated with the natural variation in Al tolerance in Arabidopsis. Our study also demonstrates the feasibility of comparative transcriptome analysis by using natural genetic variation for the identification of genes responsible for Al stress tolerance.
Volume 40(2)
Pages 249-263
Published 2017-2-1
DOI 10.1111/pce.12866
PMID 27861992
MeSH Adaptation, Physiological / drug effects Adaptation, Physiological / genetics* Aluminum / toxicity* Arabidopsis / drug effects Arabidopsis / genetics* Arabidopsis / physiology* DNA, Bacterial / genetics Ecotype* Gene Expression Profiling Gene Expression Regulation, Plant / drug effects Gene Knockout Techniques Gene Ontology Gene Regulatory Networks Genes, Plant Genetic Association Studies Plant Roots / drug effects Plant Roots / genetics Polymorphism, Single Nucleotide / genetics Principal Component Analysis Reverse Genetics Sequence Analysis, RNA Transcriptome / genetics*
IF 6.362
Times Cited 8
Arabidopsis / Cultured plant cells, genes sja