論文 - 詳細
| RRC ID | 52727 |
|---|---|
| 著者 | Okuda A, Matsusaki M, Masuda T, Urade R. |
| タイトル | Identification and characterization of GmPDIL7, a soybean ER membrane-bound protein disulfide isomerase family protein. |
| ジャーナル | FEBS J |
| Abstract |
Most proteins synthesized in the endoplasmic reticulum (ER) possess intramolecular and intermolecular disulfide bonds, which play an important role in the conformational stability and function of proteins. Hence, eukaryotic cells contain protein disulfide bond formation pathways such as the protein disulfide isomerase (PDI)-ER oxidoreductin 1 (Ero1) system in the ER lumen. In this study, we identified soybean PDIL7 (GmPDIL7), a novel soybean ER membrane-bound PDI family protein, and determined its enzymatic properties. GmPDIL7 has a putative N-terminal signal sequence, a thioredoxin domain with an active center motif (CGHC), and a putative C-terminal transmembrane region. Likewise, we demonstrated that GmPDIL7 is ubiquitously expressed in soybean tissues and is localized in the ER membrane. Furthermore, GmPDIL7 associated with other soybean PDI family proteins in vivo and GmPDIL7 mRNA was slightly upregulated under ER stress. The redox potential of recombinant GmPDIL7 expressed in Escherichia coli was -187 mV, indicating that GmPDIL7 could oxidize unfolded proteins. GmPDIL7 exhibited a dithiol oxidase activity level that was similar to other soybean PDI family proteins. However, the oxidative refolding activity of GmPDIL7 was lower than other soybean PDI family proteins. GmPDIL7 was well oxidized by GmERO1. Taken together, our results indicated that GmPDIL7 primarily plays a role as a supplier of disulfide bonds in nascent proteins for oxidative folding on the ER membrane. DATABASE:The nucleotide sequence data for the GmPDIL7 cDNA are available in the DNA Data Bank of Japan (DDBJ) databases under the accession numbers LC158001. ENZYME:Protein disulfide isomerase: EC 5.3.4.1. |
| 巻・号 | 284(3) |
| ページ | 414-428 |
| 公開日 | 2017-2-1 |
| DOI | 10.1111/febs.13984 |
| PMID | 27960051 |
| MeSH | Amino Acid Sequence Cloning, Molecular Endoplasmic Reticulum / chemistry Endoplasmic Reticulum / metabolism* Escherichia coli / genetics Escherichia coli / metabolism Gene Expression Oxidation-Reduction Plant Proteins / chemistry Plant Proteins / genetics Plant Proteins / metabolism* Protein Binding Protein Disulfide-Isomerases / chemistry Protein Disulfide-Isomerases / genetics Protein Disulfide-Isomerases / metabolism* Protein Domains Protein Sorting Signals RNA, Messenger / genetics* RNA, Messenger / metabolism Recombinant Proteins / chemistry Recombinant Proteins / genetics Recombinant Proteins / metabolism Sequence Alignment Soybeans / chemistry* Soybeans / enzymology |
| IF | 4.392 |
| 引用数 | 4 |
| リソース情報 | |
| シロイヌナズナ / 植物培養細胞・遺伝子 | rpc00051 |