論文 - 詳細
| RRC ID | 52734 |
|---|---|
| 著者 | Okuzaki Y, Kaneoka H, Nishijima KI, Murakami S, Ozawa Y, Iijima S. |
| タイトル | Molecular cloning of chicken TET family genes and role of chicken TET1 in erythropoiesis. |
| ジャーナル | Biochem Biophys Res Commun |
| Abstract |
Ten-eleven translocation (TET) methylcytosine dioxygenase has potential as an active eraser to regulate the genomic DNA methylation status. We herein cloned chicken TET (cTET) family genes, and confirmed their functions. Quantitative reverse-transcription PCR showed that cTET1 was strongly expressed in erythrocytes throughout development. This cTET1 expression pattern, together with the results of methylated or hydroxymethylated DNA immunoprecipitation, suggests that cTET1 contributes to demethylation around the promoter region of the definitive-type β-globin gene βΑ in erythroid cells. The knockdown of cTET1 in T2ECs chicken erythroid progenitor cells suppressed the induction of βΑ expression under differentiation conditions. These results suggest that cTET1 plays an important role in erythroid cell differentiation. |
| 巻・号 | 490(3) |
| ページ | 753-759 |
| 公開日 | 2017-8-26 |
| DOI | 10.1016/j.bbrc.2017.06.113 |
| PII | S0006-291X(17)31244-5 |
| 解説 | ニワトリのTET1をクロニーニングし、その機能を調べた。その結果赤血球の分化に重要な役割を持っていることが示唆された。 |
| PMID | 28642138 |
| MeSH | 5-Methylcytosine / analogs & derivatives 5-Methylcytosine / metabolism* Animals Cell Line Chickens / genetics* Chickens / physiology Cloning, Molecular* DNA Methylation Dioxygenases / genetics* Dioxygenases / metabolism Erythropoiesis* HeLa Cells Humans Multigene Family Promoter Regions, Genetic beta-Globins / genetics |
| IF | 2.985 |
| 引用数 | 0 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ニワトリ・ウズラ | |