Reference - Detail
RRC ID | 52777 |
---|---|
Author | Mun T, Małolepszy A, Sandal N, Stougaard J, Andersen SU. |
Title | User Guide for the LORE1 Insertion Mutant Resource. |
Journal | Methods Mol Biol |
Abstract |
Lotus japonicus is a model legume used in the study of plant-microbe interactions, especially in the field of biological nitrogen fixation due to its ability to enter into a symbiotic relationship with a soil bacterium, Mesorhizobium loti. The LORE1 mutant population is a valuable resource for reverse genetics in L. japonicus due to its non-transgenic nature, high tagging efficiency, and low copy count. Here, we outline a workflow for identifying, ordering, and establishing homozygous LORE1 mutant lines for a gene of interest, LjFls2, including protocols for growth and genotyping of a segregating LORE1 population. |
Volume | 1610 |
Pages | 13-23 |
Published | 2017-4-25 |
DOI | 10.1007/978-1-4939-7003-2_2 |
PMID | 28439854 |
MeSH | Fabaceae / genetics Fabaceae / microbiology Genotype Lotus / genetics* Lotus / microbiology Mutagenesis, Insertional / genetics* Mutation / genetics Plant Roots / genetics Plant Roots / microbiology Symbiosis / genetics Symbiosis / physiology |
Times Cited | 0 |
Resource | |
Lotus / Glycine | Gifu B-129 |