RRC ID 52807
著者 Nakamura S, Sato H, Tanaka R, Kusuya Y, Takahashi H, Yaguchi T.
タイトル Ribosomal subunit protein typing using matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) for the identification and discrimination of Aspergillus species.
ジャーナル BMC Microbiol
Abstract BACKGROUND:Accurate identification of Aspergillus species is a very important subject. Mass spectral fingerprinting using matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) is generally employed for the rapid identification of fungal isolates. However, the results are based on simple mass spectral pattern-matching, with no peak assignment and no taxonomic input. We propose here a ribosomal subunit protein (RSP) typing technique using MALDI-TOF MS for the identification and discrimination of Aspergillus species. The results are concluded to be phylogenetic in that they reflect the molecular evolution of housekeeping RSPs.
RESULTS:The amino acid sequences of RSPs of genome-sequenced strains of Aspergillus species were first verified and compared to compile a reliable biomarker list for the identification of Aspergillus species. In this process, we revealed that many amino acid sequences of RSPs (about 10-60%, depending on strain) registered in the public protein databases needed to be corrected or newly added. The verified RSPs were allocated to RSP types based on their mass. Peak assignments of RSPs of each sample strain as observed by MALDI-TOF MS were then performed to set RSP type profiles, which were then further processed by means of cluster analysis. The resulting dendrogram based on RSP types showed a relatively good concordance with the tree based on β-tubulin gene sequences. RSP typing was able to further discriminate the strains belonging to Aspergillus section Fumigati.
CONCLUSIONS:The RSP typing method could be applied to identify Aspergillus species, even for species within section Fumigati. The discrimination power of RSP typing appears to be comparable to conventional β-tubulin gene analysis. This method would therefore be suitable for species identification and discrimination at the strain to species level. Because RSP typing can characterize the strains within section Fumigati, this method has potential as a powerful and reliable tool in the field of clinical microbiology.
巻・号 17(1)
ページ 100
公開日 2017-4-26
DOI 10.1186/s12866-017-1009-3
PII 10.1186/s12866-017-1009-3
PMID 28441930
PMC PMC5405522
MeSH Amino Acid Sequence Aspergillus / classification Aspergillus / genetics* Aspergillus / isolation & purification* Base Sequence Cluster Analysis Evolution, Molecular Genes, Essential Molecular Typing* Mycological Typing Techniques / methods Phylogeny Ribosomal Proteins / genetics* Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods*
IF 2.989
引用数 9
リソース情報
病原真核微生物 病原真菌: IFM 53842 (= A1163), IFM 54229 (= Af293), IFM 57323, IFM 57324 (= NRRL 181), IFM 54703, IFM 47547, IFM 58399, IFM 60648, IFM 61392, IFM 62073, IFM 62096, IFM 47045, IFM 59564, IFM 60053, IFM 62093, IFM 55266, IFM 62075, IFM 62083, IFM 46973, IFM 46972, IFM 5058, IFM 51744, IFM 53868, IFM 61606, IFM 62070, IFM 62100, IFM 60676 (= NRRL 1), IFM 60677 (= NRRL 3357), IFM 59475 (=RIB 40), IFM 60678 (= FGSC A4)