RRC ID |
52850
|
著者 |
Jiang K, Otani M, Shimotakahara H, Yoon JM, Park SH, Miyaji T, Nakano T, Nakamura H, Nakajima M, Asami T.
|
タイトル |
Substituted Phthalimide AC94377 Is a Selective Agonist of the Gibberellin Receptor GID1.
|
ジャーナル |
Plant Physiol
|
Abstract |
Gibberellin (GA) is a major plant hormone that regulates plant growth and development and is widely used as a plant growth regulator in agricultural production. There is an increasing demand for function-limited GA mimics due to the limitations on the agronomical application of GA to crops, including GA's high cost of producing and its leading to the crops' lodging. AC94377, a substituted phthalimide, is a chemical that mimics the growth-regulating activity of GAs in various plants, despite its structural difference. Although AC94377 is widely studied in many weeds and crops, its mode of action as a GA mimic is largely unknown. In this study, we confirmed that AC94377 displays GA-like activities in Arabidopsis (Arabidopsis thaliana) and demonstrated that AC94377 binds to the Arabidopsis GIBBERELLIN INSENSITIVE DWARF1 (GID1) receptor (AtGID1), forms the AtGID1-AC94377-DELLA complex, and induces the degradation of DELLA protein. Our results also indicated that AC94377 is selective for a specific subtype among three AtGID1s and that the selectivity of AC94377 is attributable to a single residue at the entrance to the hydrophobic pocket of GID1. We conclude that AC94377 is a GID1 agonist with selectivity for a specific subtype of GID1, which could be further developed and used as a function-limited regulator of plant growth in both basic study and agriculture.
|
巻・号 |
173(1)
|
ページ |
825-835
|
公開日 |
2017-1-1
|
DOI |
10.1104/pp.16.00937
|
PII |
pp.16.00937
|
PMID |
27899534
|
PMC |
PMC5210710
|
MeSH |
Arabidopsis / drug effects*
Arabidopsis / metabolism
Arabidopsis Proteins / agonists*
Arabidopsis Proteins / chemistry
Arabidopsis Proteins / genetics
Arabidopsis Proteins / metabolism
Germination / drug effects
Gibberellins / metabolism
Hypocotyl / drug effects
Phthalimides / chemistry*
Phthalimides / metabolism
Phthalimides / pharmacology*
Plants, Genetically Modified
Receptors, Cell Surface / agonists*
Receptors, Cell Surface / chemistry
Receptors, Cell Surface / genetics
Receptors, Cell Surface / metabolism
Signal Transduction / drug effects
Tobacco / genetics
|
IF |
6.902
|
引用数 |
6
|
リソース情報 |
シロイヌナズナ / 植物培養細胞・遺伝子 |
psi00018
psi00021 |