RRC ID 52850
著者 Jiang K, Otani M, Shimotakahara H, Yoon JM, Park SH, Miyaji T, Nakano T, Nakamura H, Nakajima M, Asami T.
タイトル Substituted Phthalimide AC94377 Is a Selective Agonist of the Gibberellin Receptor GID1.
ジャーナル Plant Physiol
Abstract Gibberellin (GA) is a major plant hormone that regulates plant growth and development and is widely used as a plant growth regulator in agricultural production. There is an increasing demand for function-limited GA mimics due to the limitations on the agronomical application of GA to crops, including GA's high cost of producing and its leading to the crops' lodging. AC94377, a substituted phthalimide, is a chemical that mimics the growth-regulating activity of GAs in various plants, despite its structural difference. Although AC94377 is widely studied in many weeds and crops, its mode of action as a GA mimic is largely unknown. In this study, we confirmed that AC94377 displays GA-like activities in Arabidopsis (Arabidopsis thaliana) and demonstrated that AC94377 binds to the Arabidopsis GIBBERELLIN INSENSITIVE DWARF1 (GID1) receptor (AtGID1), forms the AtGID1-AC94377-DELLA complex, and induces the degradation of DELLA protein. Our results also indicated that AC94377 is selective for a specific subtype among three AtGID1s and that the selectivity of AC94377 is attributable to a single residue at the entrance to the hydrophobic pocket of GID1. We conclude that AC94377 is a GID1 agonist with selectivity for a specific subtype of GID1, which could be further developed and used as a function-limited regulator of plant growth in both basic study and agriculture.
巻・号 173(1)
ページ 825-835
公開日 2017-1-1
DOI 10.1104/pp.16.00937
PII pp.16.00937
PMID 27899534
PMC PMC5210710
MeSH Arabidopsis / drug effects* Arabidopsis / metabolism Arabidopsis Proteins / agonists* Arabidopsis Proteins / chemistry Arabidopsis Proteins / genetics Arabidopsis Proteins / metabolism Germination / drug effects Gibberellins / metabolism Hypocotyl / drug effects Phthalimides / chemistry* Phthalimides / metabolism Phthalimides / pharmacology* Plants, Genetically Modified Receptors, Cell Surface / agonists* Receptors, Cell Surface / chemistry Receptors, Cell Surface / genetics Receptors, Cell Surface / metabolism Signal Transduction / drug effects Tobacco / genetics
IF 6.902
引用数 6
リソース情報
シロイヌナズナ / 植物培養細胞・遺伝子 psi00018 psi00021