| RRC ID |
52855
|
| 著者 |
Ikeda Y, Okada Y, Sato A, Kanai T, Tomita M, Atomi H, Kanai A.
|
| タイトル |
An archaeal RNA binding protein, FAU-1, is a novel ribonuclease related to rRNA stability in Pyrococcus and Thermococcus.
|
| ジャーナル |
Sci Rep
|
| Abstract |
Ribosome biogenesis and turnover are processes necessary for cell viability and proliferation, and many kinds of proteins are known to regulate these processes. However, many questions still remain, especially in the Archaea. Generally, several ribonucleases are required to process precursor rRNAs to their mature forms, and to degrade rRNAs for quality control. Here, we found that FAU-1, which is known to be an RNA binding protein, possesses an RNase activity against precursor 5S rRNA derived from P. furiosus and T. kodakarensis in the order Thermococcales in vitro. An in vitro analysis revealed that UA sequences in the upstream of 5S rRNA were preferentially degraded by addition of FAU-1. Moreover, a fau-1 gene deletion mutant of T. kodakarensis showed a delay of exponential phase, reduction of maximum cell number and significant changes in the nucleotide sequence lengths of its 5S, 16S, and 23S rRNAs in early exponential phase. Our results suggest that FAU-1 is a potential RNase involved in rRNA stability through maturation and/or degradation processes.
|
| 巻・号 |
7(1)
|
| ページ |
12674
|
| 公開日 |
2017-10-4
|
| DOI |
10.1038/s41598-017-13062-3
|
| PII |
10.1038/s41598-017-13062-3
|
| PMID |
28978920
|
| PMC |
PMC5627344
|
| MeSH |
Archaeal Proteins / metabolism*
Base Sequence
Cell Survival / drug effects
Ions
Magnesium / pharmacology
Mutation / genetics
Pyrococcus / cytology
Pyrococcus / enzymology*
RNA Stability* / drug effects
RNA, Archaeal / metabolism*
RNA, Ribosomal, 5S / genetics
RNA-Binding Proteins / metabolism*
Ribonucleases / metabolism*
Sequence Analysis, RNA
Thermococcus / cytology
Thermococcus / enzymology*
|
| IF |
3.998
|
| 引用数 |
1
|
| リソース情報 |
| 遺伝子材料 |
pET-pfu-FAU-1-His (RDB15910). |
