論文 - 詳細
| RRC ID | 52861 |
|---|---|
| 著者 | Kim HS, Abbasi N, Choi SB. |
| タイトル | Bruno-like proteins modulate flowering time via 3' UTR-dependent decay of SOC1 mRNA. |
| ジャーナル | New Phytol |
| Abstract |
The Bruno RNA-binding protein (RBP) has been shown to initially repress the translation of oskar mRNA during Drosophila oogenesis and later to be involved in a broad range of RNA regulation. Here, we show that homologous constitutive overexpression of each of two Arabidopsis thaliana Bruno-like genes, AtBRN1 and AtBRN2, delayed the flowering time, while the atbrn1 atbrn2-3 double mutant flowered early and exhibited increased expression of APETALA1 (AP1) and LEAFY (LFY) transcripts. Crossing of 35S::AtBRNs with SOC1 101-D plants demonstrated that 35S::AtBRNs suppress an early-flowering phenotype of SOC1 101-D in which the coding sequence (CDS) with the 3' UTR of SUPPRESSOR OF OVEREXPRESSION OF CONSTANS1 (SOC1) gene is overexpressed. However, this early-flowering phenotype by SOC1 overexpression was maintained in the plants coexpressing 35S::AtBRNs and 35S::SOC1 without the 3' UTR (-3' UTR). Using yeast three-hybrid, electrophoretic mobility shift, RNA immunoprecipitation, and protoplast transient assays, we found that AtBRNs bind to the 3' UTR of SOC1 RNA and participate in mRNA decay, which was mediated by the distal region of the SOC1 3' UTR. Overall, AtBRNs repress SOC1 activity in a 3' UTR-dependent manner, thereby controlling the flowering time in Arabidopsis. |
| 巻・号 | 198(3) |
| ページ | 747-756 |
| 公開日 | 2013-5-1 |
| DOI | 10.1111/nph.12181 |
| PMID | 23437850 |
| MeSH | 3' Untranslated Regions* Arabidopsis Proteins / genetics Arabidopsis Proteins / metabolism* Drosophila Proteins / genetics Epistasis, Genetic Flowers / genetics Flowers / metabolism Flowers / physiology* Gene Expression Regulation, Plant MADS Domain Proteins / genetics* MADS Domain Proteins / metabolism* Phenotype Plants, Genetically Modified RNA Stability / genetics* RNA-Binding Proteins / genetics RNA-Binding Proteins / metabolism* Time Factors |
| IF | 8.512 |
| 引用数 | 9 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| シロイヌナズナ / 植物培養細胞・遺伝子 | pda06133 |