RRC ID 52861
Author Kim HS, Abbasi N, Choi SB.
Title Bruno-like proteins modulate flowering time via 3' UTR-dependent decay of SOC1 mRNA.
Journal New Phytol.
Abstract The Bruno RNA-binding protein (RBP) has been shown to initially repress the translation of oskar mRNA during Drosophila oogenesis and later to be involved in a broad range of RNA regulation. Here, we show that homologous constitutive overexpression of each of two Arabidopsis thaliana Bruno-like genes, AtBRN1 and AtBRN2, delayed the flowering time, while the atbrn1 atbrn2-3 double mutant flowered early and exhibited increased expression of APETALA1 (AP1) and LEAFY (LFY) transcripts. Crossing of 35S::AtBRNs with SOC1 101-D plants demonstrated that 35S::AtBRNs suppress an early-flowering phenotype of SOC1 101-D in which the coding sequence (CDS) with the 3' UTR of SUPPRESSOR OF OVEREXPRESSION OF CONSTANS1 (SOC1) gene is overexpressed. However, this early-flowering phenotype by SOC1 overexpression was maintained in the plants coexpressing 35S::AtBRNs and 35S::SOC1 without the 3' UTR (-3' UTR). Using yeast three-hybrid, electrophoretic mobility shift, RNA immunoprecipitation, and protoplast transient assays, we found that AtBRNs bind to the 3' UTR of SOC1 RNA and participate in mRNA decay, which was mediated by the distal region of the SOC1 3' UTR. Overall, AtBRNs repress SOC1 activity in a 3' UTR-dependent manner, thereby controlling the flowering time in Arabidopsis.
Volume 198(3)
Pages 747-56
Published 2013-5
DOI 10.1111/nph.12181
PMID 23437850
MeSH 3' Untranslated Regions* Arabidopsis Proteins / genetics Arabidopsis Proteins / metabolism* Drosophila Proteins / genetics Epistasis, Genetic Flowers / genetics Flowers / metabolism Flowers / physiology* Gene Expression Regulation, Plant MADS Domain Proteins / genetics* MADS Domain Proteins / metabolism* Phenotype Plants, Genetically Modified RNA Stability / genetics* RNA-Binding Proteins / genetics RNA-Binding Proteins / metabolism* Time Factors
IF 7.433
Arabidopsis / Cultured plant cells, genes pda06133