RRC ID 52865
Author Shiomi D, Toyoda A, Aizu T, Ejima F, Fujiyama A, Shini T, Kohara Y, Niki H.
Title Mutations in cell elongation genes mreB, mrdA and mrdB suppress the shape defect of RodZ-deficient cells.
Journal Mol Microbiol
Abstract RodZ interacts with MreB and both factors are required to maintain the rod shape of Escherichia coli. The assembly of MreB into filaments regulates the subcellular arrangement of a group of enzymes that synthesizes the peptidoglycan (PG) layer. However, it is still unknown how polymerization of MreB determines the rod shape of bacterial cells. Regulatory factor(s) are likely to be involved in controlling the function and dynamics of MreB. We isolated suppressor mutations to partially recover the rod shape in rodZ deletion mutants and found that some of the suppressor mutations occurred in mreB. All of the mreB mutations were in or in the vicinity of domain IA of MreB. Those mreB mutations changed the property of MreB filaments in vivo. In addition, suppressor mutations were found in the periplasmic regions in PBP2 and RodA, encoded by mrdA and mrdB genes. Similar to MreB and RodZ, PBP2 and RodA are pivotal to the cell wall elongation process. Thus, we found that mutations in domain IA of MreB and in the periplasmic domain of PBP2 and RodA can restore growth and rod shape to ΔrodZ cells, possibly by changing the requirements of MreB in the process.
Volume 87(5)
Pages 1029-44
Published 2013-3-1
DOI 10.1111/mmi.12148
PMID 23301723
PMC PMC3599482
MeSH Cytoskeletal Proteins / deficiency* Cytoskeletal Proteins / genetics Down-Regulation* Escherichia coli / cytology* Escherichia coli / genetics Escherichia coli / metabolism* Escherichia coli Proteins / genetics* Escherichia coli Proteins / metabolism Gene Deletion Gene Expression Regulation, Bacterial Membrane Proteins / genetics* Membrane Proteins / metabolism Penicillin-Binding Proteins / genetics* Penicillin-Binding Proteins / metabolism Suppression, Genetic*
IF 3.418
Times Cited 36
Prokaryotes E. coli ME9812 ME9823 ME9824 ME9825 ME9826 ME9827 ME9829 ME9830 ME9831 ME9836 ME9837 ME9838 ME9841 ME9842 ME9844 ME9845 ME9846 ME9847 ME9848 ME9849 ME9850 ME9851 ME9855 ME9856 ME9857 ME9858 ME9863 ME9864 ME9865 ME9866 ME9867 ME9868 ME9869 ME9870 ME9871 ME9872 ME9877 ME9878