RRC ID 52867
Author Shiomi D, Niki H.
Title A mutation in the promoter region of zipA, a component of the divisome, suppresses the shape defect of RodZ-deficient cells.
Journal Microbiologyopen
Abstract RodZ is important for maintaining the rod shape of Escherichia coli. Loss of RodZ causes conversion of the rod shape to a round shape and a growth rate slower than that of wild-type cells. Suppressor mutations that simultaneously restore both the growth rates and the rod shape were isolated. Most of the suppressor mutations are found in mreB, mrdA, or mrdB. One of the mutations was in the promoter region of zipA, which encodes a crucial component of the cell division machinery. In this study, we investigated the mechanism of the suppression by this mutation. ZipA was slightly but significantly increased in the suppressor cells and led to a delay in cell division. While round-shaped mreB and mrdA mutants lose cell bipolarity, we found that round-shaped rodZ mutants retained cell bipolarity. Therefore, we concluded that a delay in the completion of septation provides extra time to elongate the cell laterally so that the zipA suppressor mutant is able to recover its ovoid or rod shape. The suppression by zipA demonstrates that the regulation of timing of septation potentially contributes to the conversion of morphology in bacterial cells.
Volume 2(5)
Pages 798-810
Published 2013-10-1
DOI 10.1002/mbo3.116
PMID 23922320
PMC PMC3831641
MeSH Carrier Proteins / genetics* Carrier Proteins / metabolism Cell Cycle Proteins / genetics* Cell Cycle Proteins / metabolism Cell Division Cytoskeletal Proteins / deficiency Cytoskeletal Proteins / genetics* Escherichia coli / genetics* Escherichia coli / metabolism Escherichia coli / ultrastructure* Escherichia coli Proteins / genetics* Escherichia coli Proteins / metabolism Gene Expression Regulation, Bacterial* Membrane Proteins / genetics Membrane Proteins / metabolism Penicillin-Binding Proteins / genetics Penicillin-Binding Proteins / metabolism Promoter Regions, Genetic Suppression, Genetic* Time Factors
IF 3.142
Times Cited 8
Prokaryotes E. coli ME9828 ME9832 ME9833 ME9834 ME9835 ME9839 ME9840 ME9843 ME9852 ME9853 ME9854 ME9859 ME9860 ME9861 ME9862