RRC ID 52870
著者 Ogawa Y, Suzuki H, Sakurai N, Aoki K, Saito K, Shibata D.
タイトル Cryopreservation and metabolic profiling analysis of Arabidopsis T87 suspension-cultured cells.
ジャーナル Cryo Letters
Abstract We established a simple cryopreservation protocol for Arabidopsis T87 cells using an encapsulation-dehydration method. T87 cells were encapsulated into alginate beads containing 2 M glycerol and 0.4 M sucrose. Alginate beads containing T87 cells were dehydrated with silica gel for 2 h (to c. 0.7 g water per g dry weight followed by immersed in LN. After rewarming at 35 degree C for 3 min and 1-d incubation under continuous illumination at 22 degree C, cryopreserved T87 cells exhibited considerable regrowth. Exponentially-grown 7-d-old T87 cells regrew more vigorously (86% of control) than 14-d-old cells after cryopreservation without preculture in medium containing 0.3 M sucrose. Genetic stability of cryopreserved T87 cells was demonstrated by gas chromatography time-of-flight mass spectrometry (GC-TOF-MS) and principal component analysis (PCA). Transformed T87 cells were cryopreserved using established protocols, and GUS expression was maintained within a 2-fold variance. These results indicate that cryopreservation of T87 cells is useful for comprehensive metabolomics research and for the large scale collection of transformed cultured cell lines for functional genomics research.
巻・号 29(5)
ページ 427-36
公開日 2008-1-1
PMID 18946557
MeSH Agrobacterium tumefaciens Arabidopsis / growth & development Arabidopsis / metabolism* Cell Line Cells, Cultured Cryopreservation* Desiccation Gas Chromatography-Mass Spectrometry Genetic Vectors Glucuronidase / metabolism Metabolome* Microspheres Transformation, Genetic
IF 0.702
引用数 12
リソース情報
シロイヌナズナ / 植物培養細胞・遺伝子 rpc00008