| RRC ID |
52885
|
| 著者 |
Lee A, Asahina K, Okamoto T, Kawaguchi K, Kostsin DG, Kashiwayama Y, Takanashi K, Yazaki K, Imanaka T, Morita M.
|
| タイトル |
Role of NH2-terminal hydrophobic motif in the subcellular localization of ATP-binding cassette protein subfamily D: common features in eukaryotic organisms.
|
| ジャーナル |
Biochem Biophys Res Commun
|
| Abstract |
In mammals, four ATP-binding cassette (ABC) proteins belonging to subfamily D have been identified. ABCD1-3 possesses the NH2-terminal hydrophobic region and are targeted to peroxisomes, while ABCD4 lacking the region is targeted to the endoplasmic reticulum (ER). Based on hydropathy plot analysis, we found that several eukaryotes have ABCD protein homologs lacking the NH2-terminal hydrophobic segment (H0 motif). To investigate whether the role of the NH2-terminal H0 motif in subcellular localization is conserved across species, we expressed ABCD proteins from several species (metazoan, plant and fungi) in fusion with GFP in CHO cells and examined their subcellular localization. ABCD proteins possessing the NH2-terminal H0 motif were localized to peroxisomes, while ABCD proteins lacking this region lost this capacity. In addition, the deletion of the NH2-terminal H0 motif of ABCD protein resulted in their localization to the ER. These results suggest that the role of the NH2-terminal H0 motif in organelle targeting is widely conserved in living organisms.
|
| 巻・号 |
453(3)
|
| ページ |
612-8
|
| 公開日 |
2014-10-24
|
| DOI |
10.1016/j.bbrc.2014.09.133
|
| PII |
S0006-291X(14)01776-8
|
| PMID |
25301552
|
| MeSH |
ATP-Binding Cassette Transporters / chemistry
ATP-Binding Cassette Transporters / metabolism*
Animals
Arabidopsis / metabolism
CHO Cells
Caenorhabditis elegans / metabolism
Cricetinae
Cricetulus
Eukaryotic Cells / metabolism
Fluorescent Antibody Technique
Hydrophobic and Hydrophilic Interactions
Subcellular Fractions / metabolism*
|
| IF |
2.985
|
| 引用数 |
4
|
| リソース情報 |
| シロイヌナズナ / 植物培養細胞・遺伝子 |
pdx10506 |
