RRC ID 52885
Author Lee A, Asahina K, Okamoto T, Kawaguchi K, Kostsin DG, Kashiwayama Y, Takanashi K, Yazaki K, Imanaka T, Morita M.
Title Role of NH2-terminal hydrophobic motif in the subcellular localization of ATP-binding cassette protein subfamily D: common features in eukaryotic organisms.
Journal Biochem. Biophys. Res. Commun.
Abstract In mammals, four ATP-binding cassette (ABC) proteins belonging to subfamily D have been identified. ABCD1-3 possesses the NH2-terminal hydrophobic region and are targeted to peroxisomes, while ABCD4 lacking the region is targeted to the endoplasmic reticulum (ER). Based on hydropathy plot analysis, we found that several eukaryotes have ABCD protein homologs lacking the NH2-terminal hydrophobic segment (H0 motif). To investigate whether the role of the NH2-terminal H0 motif in subcellular localization is conserved across species, we expressed ABCD proteins from several species (metazoan, plant and fungi) in fusion with GFP in CHO cells and examined their subcellular localization. ABCD proteins possessing the NH2-terminal H0 motif were localized to peroxisomes, while ABCD proteins lacking this region lost this capacity. In addition, the deletion of the NH2-terminal H0 motif of ABCD protein resulted in their localization to the ER. These results suggest that the role of the NH2-terminal H0 motif in organelle targeting is widely conserved in living organisms.
Volume 453(3)
Pages 612-8
Published 2014-10-24
DOI 10.1016/j.bbrc.2014.09.133
PII S0006-291X(14)01776-8
PMID 25301552
MeSH ATP-Binding Cassette Transporters / chemistry ATP-Binding Cassette Transporters / metabolism* Animals Arabidopsis / metabolism CHO Cells Caenorhabditis elegans / metabolism Cricetinae Cricetulus Eukaryotic Cells / metabolism Fluorescent Antibody Technique Hydrophobic and Hydrophilic Interactions Subcellular Fractions / metabolism*
IF 2.559
Resource
Arabidopsis / Cultured plant cells, genes pdx10506