Reference - Detail
| RRC ID | 52892 |
|---|---|
| Author | Elrouby N, Bonequi MV, Porri A, Coupland G. |
| Title | Identification of Arabidopsis SUMO-interacting proteins that regulate chromatin activity and developmental transitions. |
| Journal | Proc Natl Acad Sci U S A |
| Abstract |
Posttranslational modification of proteins by small ubiquitin-like modifier (SUMO) plays essential roles in eukaryotic growth and development. Many covalently modified SUMO targets have been identified; however, the extent and significance of noncovalent interactions of SUMO with cellular proteins is poorly understood. Here, large-scale yeast two-hybrid screens repeatedly identified a surprisingly small number of proteins that interacted with three Arabidopsis SUMO isoforms. These SUMO-interacting proteins are nuclear and fall into two main categories: six histone or DNA methyltransferses or demethylases and six proteins that we show to be the evolutionary and functional homologs of SUMO-targeted ubiquitin ligases (STUbLs). The selectivity of the screen for several methylases and demethylases suggests that SUMO interaction with these proteins has a significant impact on chromatin methylation. Furthermore, the Arabidopsis STUbLs (AT-STUbLs) complemented to varying degrees the growth defects of the Schizosaccharomyces pombe STUbL mutant rfp1/rfp2, and three of them also complemented the genome integrity defects of this mutant, demonstrating that these proteins show STUbL activity. We show that one of the AT-STUbLs least related to the S. pombe protein, AT-STUbL4, has acquired a plant-specific function in the floral transition. It reduces protein levels of CYCLING DOF FACTOR 2, hence increasing transcript levels of CONSTANS and promoting flowering through the photoperiodic pathway. |
| Volume | 110(49) |
| Pages | 19956-61 |
| Published | 2013-12-3 |
| DOI | 10.1073/pnas.1319985110 |
| PII | 1319985110 |
| PMID | 24255109 |
| PMC | PMC3856783 |
| MeSH | Arabidopsis / genetics* Arabidopsis / growth & development* Arabidopsis / metabolism Arabidopsis Proteins / metabolism* Chromatin / metabolism DNA Primers / genetics Gene Library Methylation Nuclear Proteins / metabolism Two-Hybrid System Techniques Ubiquitins / metabolism* |
| IF | 9.412 |
| Times Cited | 38 |
| Altmetric score |
オルトメトリクス指標項目
|
| The most frequently cited source | X(Twitter) |
| Total number of mentions | 1 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Arabidopsis / Cultured plant cells, genes | pda11253 pda11456 |