RRC ID 53125
Author Poliner E, Farré EM, Benning C.
Title Advanced genetic tools enable synthetic biology in the oleaginous microalgae Nannochloropsis sp.
Journal Plant Cell Rep.
Abstract Nannochloropsis is a genus of fast-growing microalgae that are regularly used for biotechnology applications. Nannochloropsis species have a high triacylglycerol content and their polar lipids are rich in the omega-3 long-chain polyunsaturated fatty acid, eicosapentaenoic acid. Placed in the heterokont lineage, the Nannochloropsis genus has a complex evolutionary history. Genome sequences are available for several species, and a number of transcriptomic datasets have been produced, making this genus a facile model for comparative genomics. There is a growing interest in Nannochloropsis species as models for the study of microalga lipid metabolism and as a chassis for synthetic biology. Recently, techniques for gene stacking, and targeted gene disruption and repression in the Nannochloropsis genus have been developed. These tools enable gene-specific, mechanistic studies and have already allowed the engineering of improved Nannochloropsis strains with superior growth, or greater bioproduction.
Volume 37(10)
Pages 1383-1399
Published 2018-10
DOI 10.1007/s00299-018-2270-0
PII 10.1007/s00299-018-2270-0
PMID 29511798
MeSH Carbon / metabolism Drug Resistance, Microbial / genetics Gene Expression Regulation Light Lipid Metabolism / genetics Microalgae / classification Microalgae / genetics* Microalgae / metabolism* Photosynthesis Protein Engineering / methods Stramenopiles / classification Stramenopiles / genetics* Stramenopiles / metabolism Synthetic Biology / methods* Transcriptome
IF 2.989
Resource
Algae NIES-2145 NIES-2146