RRC ID 53147
Author Manavski N, Torabi S, Lezhneva L, Arif MA, Frank W, Meurer J.
Title HIGH CHLOROPHYLL FLUORESCENCE145 Binds to and Stabilizes the psaA 5' UTR via a Newly Defined Repeat Motif in Embryophyta.
Journal Plant Cell
Abstract The seedling-lethal Arabidopsis thaliana high chlorophyll fluorescence145 (hcf145) mutation leads to reduced stability of the plastid tricistronic psaA-psaB-rps14 mRNA and photosystem I (PSI) deficiency. Here, we genetically mapped the HCF145 gene, which encodes a plant-specific, chloroplast-localized, modular protein containing two homologous domains related to the polyketide cyclase family comprising 37 annotated Arabidopsis proteins of unknown function. Two further highly conserved and previously uncharacterized tandem repeat motifs at the C terminus, herein designated the transcript binding motif repeat (TMR) domains, confer sequence-specific RNA binding capability to HCF145. Homologous TMR motifs are often found as multiple repeats in quite diverse proteins of green and red algae and in the cyanobacterium Microcoleus sp PCC 7113 with unknown function. HCF145 represents the only TMR protein found in vascular plants. Detailed analysis of hcf145 mutants in Arabidopsis and Physcomitrella patens as well as in vivo and in vitro RNA binding assays indicate that HCF145 has been recruited in embryophyta for the stabilization of the psaA-psaB-rps14 mRNA via specific binding to its 5' untranslated region. The polyketide cyclase-related motifs support association of the TMRs to the psaA RNA, presumably pointing to a regulatory role in adjusting PSI levels according to the requirements of the plant cell.
Volume 27(9)
Pages 2600-15
Published 2015-9
DOI 10.1105/tpc.15.00234
PII tpc.15.00234
PMID 26307378
PMC PMC4815088
MeSH 5' Untranslated Regions* Alleles Amino Acid Motifs* Arabidopsis / genetics Arabidopsis Proteins / chemistry Arabidopsis Proteins / genetics* Arabidopsis Proteins / metabolism Bryophyta / genetics Chloroplasts / metabolism Embryophyta / genetics* Genetic Complementation Test Mutation Nuclear Proteins / chemistry Nuclear Proteins / genetics Nuclear Proteins / metabolism* Photosystem I Protein Complex / genetics Plants, Genetically Modified Protein Biosynthesis Repetitive Sequences, Amino Acid Ribosomal Proteins / genetics
IF 8.631
Times Cited 8
Resource
Arabidopsis / Cultured plant cells, genes pda10898