RRC ID 53169
Author Shioda N, Yabuki Y, Yamaguchi K, Onozato M, Li Y, Kurosawa K, Tanabe H, Okamoto N, Era T, Sugiyama H, Wada T, Fukunaga K.
Title Targeting G-quadruplex DNA as cognitive function therapy for ATR-X syndrome.
Journal Nat Med
Abstract Alpha-thalassemia X-linked intellectual disability (ATR-X) syndrome is caused by mutations in ATRX, which encodes a chromatin-remodeling protein. Genome-wide analyses in mouse and human cells indicate that ATRX tends to bind to G-rich sequences with a high potential to form G-quadruplexes. Here, we report that Atrx mutation induces aberrant upregulation of Xlr3b expression in the mouse brain, an outcome associated with neuronal pathogenesis displayed by ATR-X model mice. We show that ATRX normally binds to G-quadruplexes in CpG islands of the imprinted Xlr3b gene, regulating its expression by recruiting DNA methyltransferases. Xlr3b binds to dendritic mRNAs, and its overexpression inhibits dendritic transport of the mRNA encoding CaMKII-α, promoting synaptic dysfunction. Notably, treatment with 5-ALA, which is converted into G-quadruplex-binding metabolites, reduces RNA polymerase II recruitment and represses Xlr3b transcription in ATR-X model mice. 5-ALA treatment also rescues decreased synaptic plasticity and cognitive deficits seen in ATR-X model mice. Our findings suggest a potential therapeutic strategy to target G-quadruplexes and decrease cognitive impairment associated with ATR-X syndrome.
Volume 24(6)
Pages 802-813
Published 2018-6-1
DOI 10.1038/s41591-018-0018-6
PII 10.1038/s41591-018-0018-6
PMID 29785027
MeSH 3' Untranslated Regions / genetics Aminolevulinic Acid / chemistry Aminolevulinic Acid / pharmacology Aminolevulinic Acid / therapeutic use Animals Brain / metabolism Brain-Derived Neurotrophic Factor / metabolism Calcium-Calmodulin-Dependent Protein Kinase Type 2 / genetics Calcium-Calmodulin-Dependent Protein Kinase Type 2 / metabolism Cognition* Cytoplasmic Granules / metabolism Cytoskeletal Proteins / metabolism DNA / metabolism* Dendrites / metabolism G-Quadruplexes* Ligands Male Mental Retardation, X-Linked / genetics Mental Retardation, X-Linked / physiopathology* Mental Retardation, X-Linked / therapy* Mice, Inbred C57BL Nerve Tissue Proteins / metabolism Neuronal Plasticity Neurons / metabolism Nuclear Proteins / genetics Nuclear Proteins / metabolism Protein Binding RNA Polymerase II / metabolism RNA Transport RNA, Messenger / genetics RNA, Messenger / metabolism Transcription, Genetic / drug effects alpha-Thalassemia / genetics alpha-Thalassemia / physiopathology* alpha-Thalassemia / therapy*
IF 36.13
Times Cited 15
Resource
Mice RBRC04937