RRC ID 53197
Author de Souza Santos M, Salomon D, Orth K.
Title T3SS effector VopL inhibits the host ROS response, promoting the intracellular survival of Vibrio parahaemolyticus.
Journal PLoS Pathog.
Abstract The production of antimicrobial reactive oxygen species by the nicotinamide dinucleotide phosphate (NADPH) oxidase complex is an important mechanism for control of invading pathogens. Herein, we show that the gastrointestinal pathogen Vibrio parahaemolyticus counteracts reactive oxygen species (ROS) production using the Type III Secretion System 2 (T3SS2) effector VopL. In the absence of VopL, intracellular V. parahaemolyticus undergoes ROS-dependent filamentation, with concurrent limited growth. During infection, VopL assembles actin into non-functional filaments resulting in a dysfunctional actin cytoskeleton that can no longer mediate the assembly of the NADPH oxidase at the cell membrane, thereby limiting ROS production. This is the first example of how a T3SS2 effector contributes to the intracellular survival of V. parahaemolyticus, supporting the establishment of a protective intracellular replicative niche.
Volume 13(6)
Pages e1006438
Published 2017-6
DOI 10.1371/journal.ppat.1006438
PII PPATHOGENS-D-17-00284
PMID 28640881
PMC PMC5481031
MeSH Bacterial Proteins / metabolism* Caco-2 Cells Host-Pathogen Interactions / physiology* Humans Microscopy, Confocal Reactive Oxygen Species / metabolism Type III Secretion Systems / metabolism* Vibrio Infections / metabolism* Vibrio parahaemolyticus / metabolism*
IF 6.463
Times Cited 11
Resource
Pathogenic bacteria 病原細菌