| RRC ID |
53197
|
| Author |
de Souza Santos M, Salomon D, Orth K.
|
| Title |
T3SS effector VopL inhibits the host ROS response, promoting the intracellular survival of Vibrio parahaemolyticus.
|
| Journal |
PLoS Pathog
|
| Abstract |
The production of antimicrobial reactive oxygen species by the nicotinamide dinucleotide phosphate (NADPH) oxidase complex is an important mechanism for control of invading pathogens. Herein, we show that the gastrointestinal pathogen Vibrio parahaemolyticus counteracts reactive oxygen species (ROS) production using the Type III Secretion System 2 (T3SS2) effector VopL. In the absence of VopL, intracellular V. parahaemolyticus undergoes ROS-dependent filamentation, with concurrent limited growth. During infection, VopL assembles actin into non-functional filaments resulting in a dysfunctional actin cytoskeleton that can no longer mediate the assembly of the NADPH oxidase at the cell membrane, thereby limiting ROS production. This is the first example of how a T3SS2 effector contributes to the intracellular survival of V. parahaemolyticus, supporting the establishment of a protective intracellular replicative niche.
|
| Volume |
13(6)
|
| Pages |
e1006438
|
| Published |
2017-6-1
|
| DOI |
10.1371/journal.ppat.1006438
|
| PII |
PPATHOGENS-D-17-00284
|
| PMID |
28640881
|
| PMC |
PMC5481031
|
| MeSH |
Bacterial Proteins / metabolism*
Caco-2 Cells
Host-Pathogen Interactions / physiology*
Humans
Microscopy, Confocal
Reactive Oxygen Species / metabolism
Type III Secretion Systems / metabolism*
Vibrio Infections / metabolism*
Vibrio parahaemolyticus / metabolism*
|
| IF |
6.218
|
| Times Cited |
11
|
| Resource |
| Pathogenic bacteria |
病原細菌 |
