Reference - Detail
| RRC ID | 53207 |
|---|---|
| Author | Ito Y, Hasauda H, Kitajima T, Kiyono T. |
| Title | Ex vivo expansion of human cord blood hematopoietic progenitor cells using glutaraldehyde-fixed human bone marrow stromal cells. |
| Journal | J Biosci Bioeng |
| Abstract |
Human stromal cells were immortalized and fixed with glutaraldehyde to support an ex vivo expansion of human cord blood hematopoietic progenitor cells. In addition, this enabled glutaraldehyde-fixed stromal cells to be stored at 4 degrees C. Although freeze-dried glutaraldehyde-fixed stromal cells did not increase the number of the progenitor cells, the percent decrease in the number of CD34(+) cells in the presence of freeze-dried glutaraldehyde-fixed stromal cells was less than that in the absence of the stromal cells. Thus, glutaraldehyde-fixed stromal cells can serve as a stabilizing device for hematopoietic cell expansion. |
| Volume | 102(5) |
| Pages | 467-9 |
| Published | 2006-11-1 |
| DOI | 10.1263/jbb.102.467 |
| PII | S1389-1723(06)70693-4 |
| PMID | 17189177 |
| MeSH | Antigens, CD34 / analysis Bone Marrow Cells / physiology* Cells, Cultured Fetal Blood / cytology* Glutaral / pharmacology Hematopoiesis* Hematopoietic Stem Cells / cytology* Humans Stromal Cells / physiology |
| IF | 2.366 |
| Times Cited | 25 |
| WOS Category | FOOD SCIENCE & TECHNOLOGY BIOTECHNOLOGY & APPLIED MICROBIOLOGY |
| Resource | |
| Cord blood stem cells for research | |