論文 - 詳細
| RRC ID | 53240 |
|---|---|
| 著者 | Chen JX, Cipriani PG, Mecenas D, Polanowska J, Piano F, Gunsalus KC, Selbach M. |
| タイトル | In Vivo Interaction Proteomics in Caenorhabditis elegans Embryos Provides New Insights into P Granule Dynamics. |
| ジャーナル | Mol Cell Proteomics |
| Abstract |
Studying protein interactions in whole organisms is fundamental to understanding development. Here, we combine in vivo expressed GFP-tagged proteins with quantitative proteomics to identify protein-protein interactions of selected key proteins involved in early C. elegans embryogenesis. Co-affinity purification of interaction partners for eight bait proteins resulted in a pilot in vivo interaction map of proteins with a focus on early development. Our network reflects known biology and is highly enriched in functionally relevant interactions. To demonstrate the utility of the map, we looked for new regulators of P granule dynamics and found that GEI-12, a novel binding partner of the DYRK family kinase MBK-2, is a key regulator of P granule formation and germline maintenance. Our data corroborate a recently proposed model in which the phosphorylation state of GEI-12 controls P granule dynamics. In addition, we find that GEI-12 also induces granule formation in mammalian cells, suggesting a common regulatory mechanism in worms and humans. Our results show that in vivo interaction proteomics provides unique insights into animal development. |
| 巻・号 | 15(5) |
| ページ | 1642-57 |
| 公開日 | 2016-5-1 |
| DOI | 10.1074/mcp.M115.053975 |
| PII | S1535-9476(20)33603-3 |
| PMID | 26912668 |
| PMC | PMC4858945 |
| MeSH | Animals Caenorhabditis elegans / embryology* Caenorhabditis elegans / metabolism Caenorhabditis elegans Proteins / analysis* Caenorhabditis elegans Proteins / metabolism Carrier Proteins / metabolism* Chromatography, Affinity Cytoplasmic Granules / metabolism Dyrk Kinases Gene Expression Regulation, Developmental Mass Spectrometry Phosphorylation Protein Interaction Maps Protein Serine-Threonine Kinases / metabolism* Protein-Tyrosine Kinases / metabolism* Proteomics / methods* |
| IF | 4.87 |
| 引用数 | 10 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 3 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 線虫 | tm4526 tm4259 tm4343 tm3103 |