RRC ID 53240
Author Chen JX, Cipriani PG, Mecenas D, Polanowska J, Piano F, Gunsalus KC, Selbach M.
Title In Vivo Interaction Proteomics in Caenorhabditis elegans Embryos Provides New Insights into P Granule Dynamics.
Journal Mol Cell Proteomics
Abstract Studying protein interactions in whole organisms is fundamental to understanding development. Here, we combine in vivo expressed GFP-tagged proteins with quantitative proteomics to identify protein-protein interactions of selected key proteins involved in early C. elegans embryogenesis. Co-affinity purification of interaction partners for eight bait proteins resulted in a pilot in vivo interaction map of proteins with a focus on early development. Our network reflects known biology and is highly enriched in functionally relevant interactions. To demonstrate the utility of the map, we looked for new regulators of P granule dynamics and found that GEI-12, a novel binding partner of the DYRK family kinase MBK-2, is a key regulator of P granule formation and germline maintenance. Our data corroborate a recently proposed model in which the phosphorylation state of GEI-12 controls P granule dynamics. In addition, we find that GEI-12 also induces granule formation in mammalian cells, suggesting a common regulatory mechanism in worms and humans. Our results show that in vivo interaction proteomics provides unique insights into animal development.
Volume 15(5)
Pages 1642-57
Published 2016-5-1
DOI 10.1074/mcp.M115.053975
PII S1535-9476(20)33603-3
PMID 26912668
PMC PMC4858945
MeSH Animals Caenorhabditis elegans / embryology* Caenorhabditis elegans / metabolism Caenorhabditis elegans Proteins / analysis* Caenorhabditis elegans Proteins / metabolism Carrier Proteins / metabolism* Chromatography, Affinity Cytoplasmic Granules / metabolism Dyrk Kinases Gene Expression Regulation, Developmental Mass Spectrometry Phosphorylation Protein Interaction Maps Protein Serine-Threonine Kinases / metabolism* Protein-Tyrosine Kinases / metabolism* Proteomics / methods*
IF 4.87
Times Cited 10
C.elegans tm4526 tm4259 tm4343 tm3103