RRC ID |
53256
|
著者 |
Jang S, Nelson JC, Bend EG, Rodríguez-Laureano L, Tueros FG, Cartagenova L, Underwood K, Jorgensen EM, Colón-Ramos DA.
|
タイトル |
Glycolytic Enzymes Localize to Synapses under Energy Stress to Support Synaptic Function.
|
ジャーナル |
Neuron
|
Abstract |
Changes in neuronal activity create local and transient changes in energy demands at synapses. Here we discover a metabolic compartment that forms in vivo near synapses to meet local energy demands and support synaptic function in Caenorhabditis elegans neurons. Under conditions of energy stress, glycolytic enzymes redistribute from a diffuse localization in the cytoplasm to a punctate localization adjacent to synapses. Glycolytic enzymes colocalize, suggesting the ad hoc formation of a glycolysis compartment, or a "glycolytic metabolon," that can maintain local levels of ATP. Local formation of the glycolytic metabolon is dependent on presynaptic scaffolding proteins, and disruption of the glycolytic metabolon blocks the synaptic vesicle cycle, impairs synaptic recovery, and affects locomotion. Our studies indicate that under energy stress conditions, energy demands in C. elegans synapses are met locally through the assembly of a glycolytic metabolon to sustain synaptic function and behavior. VIDEO ABSTRACT.
|
巻・号 |
90(2)
|
ページ |
278-91
|
公開日 |
2016-4-20
|
DOI |
10.1016/j.neuron.2016.03.011
|
PII |
S0896-6273(16)00211-7
|
PMID |
27068791
|
PMC |
PMC4840048
|
MeSH |
Animals
Caenorhabditis elegans / cytology*
Caenorhabditis elegans / enzymology*
Caenorhabditis elegans / metabolism
Endocytosis
Hypoxia
Metabolomics
Mutation
Phosphofructokinase-1 / metabolism*
Presynaptic Terminals / enzymology*
Presynaptic Terminals / metabolism
Presynaptic Terminals / physiology*
Stress, Physiological*
Synaptic Vesicles / enzymology
Synaptic Vesicles / metabolism
|
IF |
14.415
|
引用数 |
82
|
リソース情報 |
線虫 |
tm5782
tm5613
tm408 |