Reference - Detail
| RRC ID | 53301 |
|---|---|
| Author | Wojtovich AP, Wei AY, Sherman TA, Foster TH, Nehrke K. |
| Title | Chromophore-Assisted Light Inactivation of Mitochondrial Electron Transport Chain Complex II in Caenorhabditis elegans. |
| Journal | Sci Rep |
| Abstract |
Mitochondria play critical roles in meeting cellular energy demand, in cell death, and in reactive oxygen species (ROS) and stress signaling. Most Caenorhabditis elegans loss-of-function (lf) mutants in nuclear-encoded components of the respiratory chain are non-viable, emphasizing the importance of respiratory function. Chromophore-Assisted Light Inactivation (CALI) using genetically-encoded photosensitizers provides an opportunity to determine how individual respiratory chain components contribute to physiology following acute lf. As proof-of-concept, we expressed the 'singlet oxygen generator' miniSOG as a fusion with the SDHC subunit of respiratory complex II, encoded by mev-1 in C. elegans, using Mos1-mediated Single Copy Insertion. The resulting mev-1::miniSOG transgene complemented mev-1 mutant phenotypes in kn1 missense and tm1081(lf) deletion mutants. Complex II activity was inactivated by blue light in mitochondria from strains expressing active miniSOG fusions, but not those from inactive fusions. Moreover, light-inducible phenotypes in vivo demonstrated that complex II activity is important under conditions of high energy demand, and that specific cell types are uniquely susceptible to loss of complex II. In conclusion, miniSOG-mediated CALI is a novel genetic platform for acute inactivation of respiratory chain components. Spatio-temporally controlled ROS generation will expand our understanding of how the respiratory chain and mitochondrial ROS influence whole organism physiology. |
| Volume | 6 |
| Pages | 29695 |
| Published | 2016-7-21 |
| DOI | 10.1038/srep29695 |
| PII | srep29695 |
| PMID | 27440050 |
| PMC | PMC4954975 |
| MeSH | Animals Caenorhabditis elegans / genetics Caenorhabditis elegans / growth & development Caenorhabditis elegans / metabolism* Caenorhabditis elegans Proteins / genetics* Caenorhabditis elegans Proteins / metabolism Chromophore-Assisted Light Inactivation Clutch Size Electron Transport Complex II / genetics* Electron Transport Complex II / metabolism Gene Knockdown Techniques Genetic Complementation Test Larva / genetics Larva / growth & development Larva / metabolism Mitochondria / metabolism Mutation, Missense Optogenetics |
| IF | 3.998 |
| Times Cited | 15 |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | X(Twitter) |
| Total number of mentions | 7 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| C.elegans | tm1081 |