論文 - 詳細
| RRC ID | 53346 |
|---|---|
| 著者 | Yogev S, Cooper R, Fetter R, Horowitz M, Shen K. |
| タイトル | Microtubule Organization Determines Axonal Transport Dynamics. |
| ジャーナル | Neuron |
| Abstract |
Axonal microtubule (MT) arrays are the major cytoskeleton substrate for cargo transport. How MT organization, i.e., polymer length, number, and minus-end spacing, is regulated and how it impinges on axonal transport are unclear. We describe a method for analyzing neuronal MT organization using light microscopy. This method circumvents the need for electron microscopy reconstructions and is compatible with live imaging of cargo transport and MT dynamics. Examination of a C. elegans motor neuron revealed how age, MT-associated proteins, and signaling pathways control MT length, minus-end spacing, and coverage. In turn, MT organization determines axonal transport progression: cargoes pause at polymer termini, suggesting that switching MT tracks is rate limiting for efficient transport. Cargo run length is set by MT length, and higher MT coverage correlates with shorter pauses. These results uncover the principles and mechanisms of neuronal MT organization and its regulation of axonal cargo transport. |
| 巻・号 | 92(2) |
| ページ | 449-460 |
| 公開日 | 2016-10-19 |
| DOI | 10.1016/j.neuron.2016.09.036 |
| PII | S0896-6273(16)30637-7 |
| PMID | 27764672 |
| PMC | PMC5432135 |
| MeSH | Animals Axonal Transport* Caenorhabditis elegans Cytoskeleton / metabolism* Dyneins / metabolism Kinesins / metabolism Microscopy Microtubule-Associated Proteins / metabolism* Microtubules / metabolism* Microtubules / ultrastructure Motor Neurons / metabolism* Motor Neurons / ultrastructure Polymers / metabolism* Signal Transduction Time-Lapse Imaging |
| IF | 14.415 |
| 引用数 | 42 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 13 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 線虫 | tm1357 tm4830 tm2143 tm3737 tm3124 tm5775 |