| RRC ID |
53354
|
| Author |
Ujisawa T, Ohta A, Uda-Yagi M, Kuhara A.
|
| Title |
Diverse Regulation of Temperature Sensation by Trimeric G-Protein Signaling in Caenorhabditis elegans.
|
| Journal |
PLoS One
|
| Abstract |
Temperature sensation by the nervous system is essential for life and proliferation of animals. The molecular-physiological mechanisms underlying temperature signaling have not been fully elucidated. We show here that diverse regulatory machinery underlies temperature sensation through trimeric G-protein signaling in the nematode Caenorhabditis elegans. Molecular-genetic studies demonstrated that cold tolerance is regulated by additive functions of three Gα proteins in a temperature-sensing neuron, ASJ, which is also known to be a light-sensing neuron. Optical recording of calcium concentration in ASJ upon temperature-changes demonstrated that three Gα proteins act in different aspects of temperature signaling. Calcium concentration changes in ASJ upon temperature change were unexpectedly decreased in a mutant defective in phosphodiesterase, which is well known as a negative regulator of calcium increase. Together, these data demonstrate commonalities and differences in the molecular components concerned with light and temperature signaling in a single sensory neuron.
|
| Volume |
11(10)
|
| Pages |
e0165518
|
| Published |
2016-1-1
|
| DOI |
10.1371/journal.pone.0165518
|
| PII |
PONE-D-16-19856
|
| PMID |
27788246
|
| PMC |
PMC5082853
|
| MeSH |
Adaptation, Physiological
Animals
Caenorhabditis elegans / metabolism*
Caenorhabditis elegans / physiology
Calcium / metabolism
Cold Temperature
GTP-Binding Proteins / metabolism*
Germ Cells
Microscopy, Confocal
Signal Transduction*
Temperature*
|
| IF |
2.74
|
| Times Cited |
5
|
| Resource |
| C.elegans |
tm3098 |
