RRC ID 53385
著者 Erdelyi P, Wang X, Suleski M, Wicky C.
タイトル A Network of Chromatin Factors Is Regulating the Transition to Postembryonic Development in Caenorhabditis elegans.
ジャーナル G3 (Bethesda)
Abstract Mi2 proteins are evolutionarily conserved, ATP-dependent chromatin remodelers of the CHD family that play key roles in stem cell differentiation and reprogramming. In Caenorhabditis elegans, the let-418 gene encodes one of the two Mi2 homologs, which is part of at least two chromatin complexes, namely the Nucleosome Remodeling and histone Deacetylase (NuRD) complex and the MEC complex, and functions in larval development, vulval morphogenesis, lifespan regulation, and cell fate determination. To explore the mechanisms involved in the action of LET-418/Mi2, we performed a genome-wide RNA interference (RNAi) screen for suppressors of early larval arrest associated with let-418 mutations. We identified 29 suppressor genes, of which 24 encode chromatin regulators, mostly orthologs of proteins present in transcriptional activator complexes. The remaining five genes vary broadly in their predicted functions. All suppressor genes could suppress multiple aspects of the let-418 phenotype, including developmental arrest and ectopic expression of germline genes in the soma. Analysis of available transcriptomic data and quantitative PCR revealed that LET-418 and the suppressors of early larval arrest are regulating common target genes. These suppressors might represent direct competitors of LET-418 complexes for chromatin regulation of crucial genes involved in the transition to postembryonic development.
巻・号 7(2)
ページ 343-353
公開日 2017-2-9
DOI 10.1534/g3.116.037747
PII g3.116.037747
PMID 28007841
PMC PMC5295584
MeSH Animals Caenorhabditis elegans / genetics* Caenorhabditis elegans / growth & development Caenorhabditis elegans Proteins / genetics* Chromatin / genetics Chromatin Assembly and Disassembly / genetics DNA-Binding Proteins / genetics* Embryonic Development / genetics* Genome Larva / genetics* Larva / growth & development Mi-2 Nucleosome Remodeling and Deacetylase Complex / genetics Phenotype RNA Interference
IF 2.781
引用数 2
リソース情報
線虫 tm2467