RRC ID 53403
Author Lee D, Lee H, Kim N, Lim DS, Lee J.
Title Regulation of a hitchhiking behavior by neuronal insulin and TGF-β signaling in the nematode Caenorhabditis elegans.
Journal Biochem Biophys Res Commun
Abstract Free-living nematode Caenorhabditis elegans exhibits various behaviors to adapt to the fluctuating environment. When early larvae of C. elegans experience the harsh environmental condition, they develop to an alternative developmental stage called dauer, which shows nictation, a stage-specific waving behavior. Nictation enables dauers to attach to more mobile animals, which helps them disperse to other habitats beyond physical barriers. However, underlying molecular mechanisms that regulate nictation behavior are largely unknown. In this study, we show that insulin signaling and transforming growth beta (TGF-β) signaling, the two major parallel signaling pathways that mediate dauer development, are involved in the regulation of dauer-specific nictation behavior. Genetic analysis revealed that downregulation of insulin signaling enhanced nictation behavior. Heat-shock induced rescue experiments showed that the action period of the insulin signaling is before dauer formation. Surprisingly, lowering of TGF-β signaling inhibited the normal performance of nictation, suggesting that TGF-β signaling acts in an opposite way from that for dauer formation. Cell-specific rescue experiments revealed that two signaling pathways act in the nervous system and an epistasis experiment showed that TGF-β signaling is epistatic to insulin signaling. Taken together, we propose that the neuroendocrinal insulin signaling and TGF-β signaling regulate nictation behavior during development in response to environmental conditions.
Volume 484(2)
Pages 323-330
Published 2017-3-4
DOI 10.1016/j.bbrc.2017.01.113
PII S0006-291X(17)30173-0
PMID 28131836
MeSH Animals Caenorhabditis elegans / metabolism* Caenorhabditis elegans Proteins / metabolism Insulin / metabolism* Neurons / metabolism* Signal Transduction* Transforming Growth Factor beta / metabolism* p21-Activated Kinases / metabolism
IF 2.985
Times Cited 6
C.elegans tm1888 tm4467 tm3608 tm3620 tm2560 tm2416 tm2001 tm4144 tm3618 tm3498