RRC ID 53404
Author Rojo Romanos T, Petersen JG, Pocock R.
Title Control of Neuropeptide Expression by Parallel Activity-dependent Pathways in Caenorhabditis elegans.
Journal Sci Rep
Abstract Monitoring of neuronal activity within circuits facilitates integrated responses and rapid changes in behavior. We have identified a system in Caenorhabditis elegans where neuropeptide expression is dependent on the ability of the BAG neurons to sense carbon dioxide. In C. elegans, CO2 sensing is predominantly coordinated by the BAG-expressed receptor-type guanylate cyclase GCY-9. GCY-9 binding to CO2 causes accumulation of cyclic GMP and opening of the cGMP-gated TAX-2/TAX-4 cation channels; provoking an integrated downstream cascade that enables C. elegans to avoid high CO2. Here we show that cGMP regulation by GCY-9 and the PDE-1 phosphodiesterase controls BAG expression of a FMRFamide-related neuropeptide FLP-19 reporter (flp-19::GFP). This regulation is specific for CO2-sensing function of the BAG neurons, as loss of oxygen sensing function does not affect flp-19::GFP expression. We also found that expression of flp-19::GFP is controlled in parallel to GCY-9 by the activity-dependent transcription factor CREB (CRH-1) and the cAMP-dependent protein kinase (KIN-2) signaling pathway. We therefore show that two parallel pathways regulate neuropeptide gene expression in the BAG sensory neurons: the ability to sense changes in carbon dioxide and CREB transcription factor. Such regulation may be required in particular environmental conditions to enable sophisticated behavioral decisions to be performed.
Volume 7
Pages 38734
Published 2017-1-31
DOI 10.1038/srep38734
PII srep38734
PMID 28139692
PMC PMC5282578
MeSH Animals Caenorhabditis elegans / physiology* Caenorhabditis elegans Proteins / metabolism Carbon Dioxide / metabolism* Cyclic GMP / metabolism Cyclic Nucleotide Phosphodiesterases, Type 1 / metabolism Gene Expression Regulation* Neuropeptides / biosynthesis* Receptors, Guanylate Cyclase-Coupled / metabolism Sensory Receptor Cells / physiology*
IF 4.011
Times Cited 10
Resource
C.elegans tm2816