論文 - 詳細
| RRC ID | 53439 |
|---|---|
| 著者 | Horsman JW, Miller DL. |
| タイトル | Mitochondrial Sulfide Quinone Oxidoreductase Prevents Activation of the Unfolded Protein Response in Hydrogen Sulfide. |
| ジャーナル | J Biol Chem |
| Abstract |
Hydrogen sulfide (H2S) is an endogenously produced gaseous molecule with important roles in cellular signaling. In mammals, exogenous H2S improves survival of ischemia/reperfusion. We have previously shown that exposure to H2S increases the lifespan and thermotolerance in Caenorhabditis elegans, and improves protein homeostasis in low oxygen. The mitochondrial SQRD-1 (sulfide quinone oxidoreductase) protein is a highly conserved enzyme involved in H2S metabolism. SQRD-1 is generally considered important to detoxify H2S. Here, we show that SQRD-1 is also required to maintain protein translation in H2S. In sqrd-1 mutant animals, exposure to H2S leads to phosphorylation of eIF2α and inhibition of protein synthesis. In contrast, global protein translation is not altered in wild-type animals exposed to lethally high H2S or in hif-1(ia04) mutants that die when exposed to low H2S. We demonstrate that both gcn-2 and pek-1 kinases are involved in the H2S-induced phosphorylation of eIF2α. Both ER and mitochondrial stress responses are activated in sqrd-1 mutant animals exposed to H2S, but not in wild-type animals. We speculate that SQRD-1 activity in H2S may coordinate proteostasis responses in multiple cellular compartments. |
| 巻・号 | 291(10) |
| ページ | 5320-5 |
| 公開日 | 2016-3-4 |
| DOI | 10.1074/jbc.M115.697102 |
| PII | S0021-9258(20)43167-9 |
| PMID | 26677221 |
| PMC | PMC4777863 |
| MeSH | Animals Caenorhabditis elegans / metabolism Caenorhabditis elegans Proteins / genetics Caenorhabditis elegans Proteins / metabolism Carrier Proteins / genetics Carrier Proteins / metabolism Cell Cycle Proteins / genetics Cell Cycle Proteins / metabolism Eukaryotic Initiation Factor-2 / metabolism Hydrogen Sulfide / metabolism* Hydrogen Sulfide / toxicity MAP Kinase Kinase 1 / genetics MAP Kinase Kinase 1 / metabolism Oxidoreductases Acting on Sulfur Group Donors / genetics Oxidoreductases Acting on Sulfur Group Donors / metabolism* Transcription Factors / genetics Transcription Factors / metabolism Unfolded Protein Response* |
| IF | 4.238 |
| 引用数 | 5 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 6 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 線虫 | tm3378 |