Reference - Detail
| RRC ID | 53461 |
|---|---|
| Author | Xu S, Chisholm AD. |
| Title | Highly efficient optogenetic cell ablation in C. elegans using membrane-targeted miniSOG. |
| Journal | Sci Rep |
| Abstract |
The genetically encoded photosensitizer miniSOG (mini Singlet Oxygen Generator) can be used to kill cells in C. elegans. miniSOG generates the reactive oxygen species (ROS) singlet oxygen after illumination with blue light. Illumination of neurons expressing miniSOG targeted to the outer mitochondrial membrane (mito-miniSOG) causes neuronal death. To enhance miniSOG's efficiency as an ablation tool in multiple cell types we tested alternative targeting signals. We find that membrane targeted miniSOG allows highly efficient cell killing. When combined with a point mutation that increases miniSOG's ROS generation, membrane targeted miniSOG can ablate neurons in less than one tenth the time of mito-miniSOG. We extend the miniSOG ablation technique to non-neuronal tissues, revealing an essential role for the epidermis in locomotion. These improvements expand the utility and throughput of optogenetic cell ablation in C. elegans. |
| Volume | 6 |
| Pages | 21271 |
| Published | 2016-2-10 |
| DOI | 10.1038/srep21271 |
| PII | srep21271 |
| PMID | 26861262 |
| PMC | PMC4748272 |
| MeSH | Ablation Techniques* Animals Caenorhabditis elegans / drug effects* Cell Death / drug effects* Epidermis / metabolism Light Membranes / metabolism* Muscle Cells / metabolism Neurons / metabolism* Optogenetics / methods Photosensitizing Agents / chemistry* Singlet Oxygen / pharmacology* |
| IF | 3.998 |
| Times Cited | 29 |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | X(Twitter) |
| Total number of mentions | 3 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| C.elegans | tm776 |