論文 - 詳細
| RRC ID | 53478 |
|---|---|
| 著者 | Knutson AK, Rechtsteiner A, Strome S. |
| タイトル | Reevaluation of whether a soma-to-germ-line transformation extends lifespan in Caenorhabditis elegans. |
| ジャーナル | Proc Natl Acad Sci U S A |
| Abstract |
The germ lineage is considered to be immortal. In the quest to extend lifespan, a possible strategy is to drive germ-line traits in somatic cells, to try to confer some of the germ lineage's immortality on the somatic body. Notably, a study in Caenorhabditis elegans suggested that expression of germ-line genes in the somatic cells of long-lived daf-2 mutants confers some of daf-2's long lifespan. Specifically, mRNAs encoding components of C. elegans germ granules (P granules) were up-regulated in daf-2 mutant worms, and knockdown of individual P-granule and other germ-line genes in daf-2 young adults modestly reduced their lifespan. We investigated the contribution of a germ-line program to daf-2's long lifespan and also tested whether other mutants known to express germ-line genes in their somatic cells are long-lived. Our key findings are as follows. (i) We could not detect P-granule proteins in the somatic cells of daf-2 mutants by immunostaining or by expression of a P-granule transgene. (ii) Whole-genome transcript profiling of animals lacking a germ line revealed that germ-line transcripts are not up-regulated in the soma of daf-2 worms compared with the soma of control worms. (iii) Simultaneous removal of multiple P-granule proteins or the entire germ-line program from daf-2 worms did not reduce their lifespan. (iv) Several mutants that robustly express a broad spectrum of germ-line genes in their somatic cells are not long-lived. Together, our findings argue against the hypothesis that acquisition of a germ-cell program in somatic cells increases lifespan and contributes to daf-2's long lifespan. |
| 巻・号 | 113(13) |
| ページ | 3591-6 |
| 公開日 | 2016-3-29 |
| DOI | 10.1073/pnas.1523402113 |
| PII | 1523402113 |
| PMID | 26976573 |
| PMC | PMC4822628 |
| MeSH | Animals Caenorhabditis elegans / genetics* Caenorhabditis elegans Proteins / genetics Gene Expression Profiling Gene Knockdown Techniques Genes, Helminth Germ-Line Mutation Longevity / genetics RNA Interference Receptor, Insulin / genetics |
| IF | 9.412 |
| 引用数 | 5 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 13 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 線虫 | tm1490 |