RRC ID 53519
Author Gruner M, Grubbs J, McDonagh A, Valdes D, Winbush A, van der Linden AM.
Title Cell-Autonomous and Non-Cell-Autonomous Regulation of a Feeding State-Dependent Chemoreceptor Gene via MEF-2 and bHLH Transcription Factors.
Journal PLoS Genet
Abstract Food and feeding-state dependent changes in chemoreceptor gene expression may allow Caenorhabditis elegans to modify their chemosensory behavior, but the mechanisms essential for these expression changes remain poorly characterized. We had previously shown that expression of a feeding state-dependent chemoreceptor gene, srh-234, in the ADL sensory neuron of C. elegans is regulated via the MEF-2 transcription factor. Here, we show that MEF-2 acts together with basic helix-loop-helix (bHLH) transcription factors to regulate srh-234 expression as a function of feeding state. We identify a cis-regulatory MEF2 binding site that is necessary and sufficient for the starvation-induced down regulation of srh-234 expression, while an E-box site known to bind bHLH factors is required to drive srh-234 expression in ADL. We show that HLH-2 (E/Daughterless), HLH-3 and HLH-4 (Achaete-scute homologs) act in ADL neurons to regulate srh-234 expression. We further demonstrate that the expression levels of srh-234 in ADL neurons are regulated remotely by MXL-3 (Max-like 3 homolog) and HLH-30 (TFEB ortholog) acting in the intestine, which is dependent on insulin signaling functioning specifically in ADL neurons. We also show that this intestine-to-neuron feeding-state regulation of srh-234 involves a subset of insulin-like peptides. These results combined suggest that chemoreceptor gene expression is regulated by both cell-autonomous and non-cell-autonomous transcriptional mechanisms mediated by MEF2 and bHLH factors, which may allow animals to fine-tune their chemosensory responses in response to changes in their feeding state.
Volume 12(8)
Pages e1006237
Published 2016-8-1
DOI 10.1371/journal.pgen.1006237
PMID 27487365
PMC PMC4972359
MeSH Animals Animals, Genetically Modified Basic Helix-Loop-Helix Transcription Factors / biosynthesis Basic Helix-Loop-Helix Transcription Factors / genetics* Binding Sites Caenorhabditis elegans / genetics Caenorhabditis elegans / growth & development Caenorhabditis elegans Proteins / biosynthesis Caenorhabditis elegans Proteins / genetics* Chemoreceptor Cells / metabolism* Gene Expression Regulation, Developmental Receptors, Neuropeptide / biosynthesis Receptors, Neuropeptide / genetics* Receptors, Pituitary Hormone-Regulating Hormone / biosynthesis Receptors, Pituitary Hormone-Regulating Hormone / genetics* Sensory Receptor Cells / metabolism Signal Transduction / genetics Transcription Factors / biosynthesis Transcription Factors / genetics*
IF 5.175
Times Cited 8
C.elegans tm1768 tm299 tm1978 tm1983 tm2308