論文 - 詳細
| RRC ID | 53530 |
|---|---|
| 著者 | Nath RD, Chow ES, Wang H, Schwarz EM, Sternberg PW. |
| タイトル | C. elegans Stress-Induced Sleep Emerges from the Collective Action of Multiple Neuropeptides. |
| ジャーナル | Curr Biol |
| Abstract |
The genetic basis of sleep regulation remains poorly understood. In C. elegans, cellular stress induces sleep through epidermal growth factor (EGF)-dependent activation of the EGF receptor in the ALA neuron. The downstream mechanism by which this neuron promotes sleep is unknown. Single-cell RNA sequencing of ALA reveals that the most highly expressed, ALA-enriched genes encode neuropeptides. Here we have systematically investigated the four most highly enriched neuropeptides: flp-7, nlp-8, flp-24, and flp-13. When individually removed by null mutation, these peptides had little or no effect on stress-induced sleep. However, stress-induced sleep was abolished in nlp-8; flp-24; flp-13 triple-mutant animals, indicating that these neuropeptides work collectively in controlling stress-induced sleep. We tested the effect of overexpression of these neuropeptide genes on five behaviors modulated during sleep-pharyngeal pumping, defecation, locomotion, head movement, and avoidance response to an aversive stimulus-and we found that, if individually overexpressed, each of three neuropeptides (nlp-8, flp-24, or flp-13) induced a different suite of sleep-associated behaviors. These overexpression results raise the possibility that individual components of sleep might be specified by individual neuropeptides or combinations of neuropeptides. |
| 巻・号 | 26(18) |
| ページ | 2446-2455 |
| 公開日 | 2016-9-26 |
| DOI | 10.1016/j.cub.2016.07.048 |
| PII | S0960-9822(16)30841-7 |
| PMID | 27546573 |
| PMC | PMC5694219 |
| MeSH | Animals Caenorhabditis elegans / genetics Caenorhabditis elegans / physiology* Caenorhabditis elegans Proteins / genetics* Caenorhabditis elegans Proteins / metabolism Neuropeptides / genetics* Neuropeptides / metabolism Sleep / genetics* Stress, Physiological* |
| IF | 9.601 |
| 引用数 | 33 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 26 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 10.0 |
| リソース情報 | |
| 線虫 | tm2427 |