RRC ID 53530
Author Nath RD, Chow ES, Wang H, Schwarz EM, Sternberg PW.
Title C. elegans Stress-Induced Sleep Emerges from the Collective Action of Multiple Neuropeptides.
Journal Curr Biol
Abstract The genetic basis of sleep regulation remains poorly understood. In C. elegans, cellular stress induces sleep through epidermal growth factor (EGF)-dependent activation of the EGF receptor in the ALA neuron. The downstream mechanism by which this neuron promotes sleep is unknown. Single-cell RNA sequencing of ALA reveals that the most highly expressed, ALA-enriched genes encode neuropeptides. Here we have systematically investigated the four most highly enriched neuropeptides: flp-7, nlp-8, flp-24, and flp-13. When individually removed by null mutation, these peptides had little or no effect on stress-induced sleep. However, stress-induced sleep was abolished in nlp-8; flp-24; flp-13 triple-mutant animals, indicating that these neuropeptides work collectively in controlling stress-induced sleep. We tested the effect of overexpression of these neuropeptide genes on five behaviors modulated during sleep-pharyngeal pumping, defecation, locomotion, head movement, and avoidance response to an aversive stimulus-and we found that, if individually overexpressed, each of three neuropeptides (nlp-8, flp-24, or flp-13) induced a different suite of sleep-associated behaviors. These overexpression results raise the possibility that individual components of sleep might be specified by individual neuropeptides or combinations of neuropeptides.
Volume 26(18)
Pages 2446-2455
Published 2016-9-26
DOI 10.1016/j.cub.2016.07.048
PII S0960-9822(16)30841-7
PMID 27546573
PMC PMC5694219
MeSH Animals Caenorhabditis elegans / genetics Caenorhabditis elegans / physiology* Caenorhabditis elegans Proteins / genetics* Caenorhabditis elegans Proteins / metabolism Neuropeptides / genetics* Neuropeptides / metabolism Sleep / genetics* Stress, Physiological*
IF 9.601
Times Cited 33
C.elegans tm2427