論文 - 詳細
| RRC ID | 53550 |
|---|---|
| 著者 | Wiedermannová J, Sudzinová P, Kovaľ T, Rabatinová A, Šanderova H, Ramaniuk O, Rittich Š, Dohnálek J, Fu Z, Halada P, Lewis P, Krásny L. |
| タイトル | Characterization of HelD, an interacting partner of RNA polymerase from Bacillus subtilis. |
| ジャーナル | Nucleic Acids Res |
| Abstract |
Bacterial RNA polymerase (RNAP) is an essential multisubunit protein complex required for gene expression. Here, we characterize YvgS (HelD) from Bacillus subtilis, a novel binding partner of RNAP. We show that HelD interacts with RNAP-core between the secondary channel of RNAP and the alpha subunits. Importantly, we demonstrate that HelD stimulates transcription in an ATP-dependent manner by enhancing transcriptional cycling and elongation. We demonstrate that the stimulatory effect of HelD can be amplified by a small subunit of RNAP, delta. In vivo, HelD is not essential but it is required for timely adaptations of the cell to changing environment. In summary, this study establishes HelD as a valid component of the bacterial transcription machinery. |
| 巻・号 | 42(8) |
| ページ | 5151-63 |
| 公開日 | 2014-4-1 |
| DOI | 10.1093/nar/gku113 |
| PII | gku113 |
| PMID | 24520113 |
| PMC | PMC4005671 |
| MeSH | Adenosine Triphosphate / metabolism Bacillus subtilis / enzymology* Bacillus subtilis / genetics Bacterial Proteins / isolation & purification Bacterial Proteins / metabolism* DNA / metabolism DNA-Directed RNA Polymerases / chemistry DNA-Directed RNA Polymerases / isolation & purification DNA-Directed RNA Polymerases / metabolism* Phenotype Transcription Elongation, Genetic Transcription, Genetic* |
| IF | 11.502 |
| 引用数 | 20 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 3 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 原核生物(大腸菌) | |