Reference - Detail
| RRC ID | 53554 |
|---|---|
| Author | Feng X, Ding Y, Xian M, Xu X, Zhang R, Zhao G. |
| Title | Production of optically pure d-lactate from glycerol by engineered Klebsiella pneumoniae strain. |
| Journal | Bioresour Technol |
| Abstract |
In this study, glycerol was used to produce optically pure d-lactate by engineered Klebsiella pneumoniae strain. In the recombinant strain, d-lactate dehydrogenase LdhA was overexpressed, and two genes, dhaT and yqhD for biosynthesis of main byproduct 1,3-propanediol, were knocked out. To further improve d-lactate production, the culture condition was optimized and the results demonstrated that aeration rate played an important role in d-lactate production. In microaerobic fed-batch fermentation, the engineered strain accumulated 142.1g/L optically pure d-lactate with a yield of 0.82g/g glycerol, which represented the highest d-lactate production from glycerol so far. This study showed that K. pneumoniae strain has high efficiency to convert glycerol into d-lactate and high potentiality in utilization of crude glycerol from biodiesel industry. |
| Volume | 172 |
| Pages | 269-275 |
| Published | 2014-11-1 |
| DOI | 10.1016/j.biortech.2014.09.074 |
| PII | S0960-8524(14)01333-9 |
| PMID | 25270041 |
| MeSH | Fermentation Glycerol / metabolism* Klebsiella pneumoniae / metabolism* Lactic Acid / biosynthesis* Metabolic Engineering* Nitrogen Compounds Oxidation-Reduction |
| IF | 7.539 |
| Times Cited | 22 |
| Altmetric score |
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| The most frequently cited source | Patent(IFI CLAIMS) |
| Total number of mentions | 7 |
| Altmetric score changes over past 6months | 3.0 |
| Resource | |
| Prokaryotes E. coli | |