| RRC ID |
53563
|
| 著者 |
Ishihama A, Kori A, Koshio E, Yamada K, Maeda H, Shimada T, Makinoshima H, Iwata A, Fujita N.
|
| タイトル |
Intracellular concentrations of 65 species of transcription factors with known regulatory functions in Escherichia coli.
|
| ジャーナル |
J Bacteriol
|
| Abstract |
The expression pattern of the Escherichia coli genome is controlled in part by regulating the utilization of a limited number of RNA polymerases among a total of its approximately 4,600 genes. The distribution pattern of RNA polymerase changes from modulation of two types of protein-protein interactions: the interaction of core RNA polymerase with seven species of the sigma subunit for differential promoter recognition and the interaction of RNA polymerase holoenzyme with about 300 different species of transcription factors (TFs) with regulatory functions. We have been involved in the systematic search for the target promoters recognized by each sigma factor and each TF using the newly developed Genomic SELEX system. In parallel, we developed the promoter-specific (PS)-TF screening system for identification of the whole set of TFs involved in regulation of each promoter. Understanding the regulation of genome transcription also requires knowing the intracellular concentrations of the sigma subunits and TFs under various growth conditions. This report describes the intracellular levels of 65 species of TF with known function in E. coli K-12 W3110 at various phases of cell growth and at various temperatures. The list of intracellular concentrations of the sigma factors and TFs provides a community resource for understanding the transcription regulation of E. coli under various stressful conditions in nature.
|
| 巻・号 |
196(15)
|
| ページ |
2718-27
|
| 公開日 |
2014-8-1
|
| DOI |
10.1128/JB.01579-14
|
| PII |
JB.01579-14
|
| PMID |
24837290
|
| PMC |
PMC4135669
|
| MeSH |
Adaptation, Physiological
Animals
Antibodies, Bacterial / biosynthesis
Escherichia coli / genetics
Escherichia coli / growth & development
Escherichia coli / immunology
Escherichia coli / metabolism*
Escherichia coli Proteins / genetics
Escherichia coli Proteins / isolation & purification
Escherichia coli Proteins / metabolism
Gene Expression Regulation, Bacterial*
Genome, Bacterial / genetics*
Intracellular Space
Promoter Regions, Genetic / genetics
Rabbits
Sequence Deletion
Sigma Factor / genetics
Sigma Factor / isolation & purification
Sigma Factor / metabolism
Stress, Physiological
Temperature
Transcription Factors / genetics
Transcription Factors / immunology
Transcription Factors / isolation & purification
Transcription Factors / metabolism*
|
| IF |
3.006
|
| 引用数 |
52
|
| リソース情報 |
| 原核生物(大腸菌) |
|
